Book Volume 287 2004

Epigenetics Protocols

Editors:

ISBN: 978-1-58829-336-7 (Print) 978-1-59259-828-1 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xi

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    Protocol

    Pages 1-8

    Methods of Epigenetic Analysis

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    Protocol

    Pages 9-19

    Chromatin Immunoprecipitation Assays

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    Protocol

    Pages 21-44

    Native Chromatin Immunoprecipitation

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    Protocol

    Pages 45-52

    Q-PCR in Combination With ChIP Assays to Detect Changes in Chromatin Acetylation

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    Protocol

    Pages 53-63

    Restriction Endonuclease Accessibility as a Determinant of Altered Chromatin Structure

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    Protocol

    Pages 65-75

    Measuring Changes in Chromatin Using Micrococcal Nuclease

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    Protocol

    Pages 77-86

    DNaseI Hypersensitivity Analysis of Chromatin Structure

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    Protocol

    Pages 87-97

    Inhibition of Histone Deacetylases

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    Protocol

    Pages 99-120

    Site-Specific Analysis of Histone Methylation and Acetylation

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    Protocol

    Pages 121-136

    Analysis of Mammalian Telomere Position Effect

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    Protocol

    Pages 137-149

    Activity Assays for Poly-ADP Ribose Polymerase

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    Protocol

    Pages 151-168

    Multigenerational Selection and Detection of Altered Histone Acetylation and Methylation Patterns

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    Protocol

    Pages 169-179

    Profiling DNA Methylation by Bisulfite Genomic Sequencing

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    Protocol

    Pages 181-193

    Methylation-Sensitive Single-Strand Conformation Analysis

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    Protocol

    Pages 195-205

    SIRPH Analysis

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    Protocol

    Pages 207-218

    Real-Time PCR-Based Assay for Quantitative Determination of Methylation Status

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    Protocol

    Pages 219-231

    Denaturing Gradient Gel Electrophoresis to Detect Methylation Changes in DNA

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    Protocol

    Pages 233-249

    Photocrosslinking Oligonucleotide Hybridization Assay for Concurrent Gene Dosage and CpG Methylation Analysis

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    Protocol

    Pages 251-260

    Methylation-Specific Oligonucleotide Microarray

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    Protocol

    Pages 261-271

    Methylation-Specific PCR In Situ Hybridization

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