Signal Transduction Protocols

  • Robert C. Dickson
  • Michael D. Mendenhall

Part of the Methods in Molecular Biology book series (MIMB, volume 284)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Elaine A. Elion, Yunmei Wang
    Pages 1-14
  3. Maofu Fu, Chenguang Wang, Xueping Zhang, Richard G. Pestell
    Pages 15-36
  4. Yukihito Kabuyama, Kirsi K. Polvinen, Katheryn A. Resing, Natalie G. Ahn
    Pages 37-49
  5. Daniel J. Noonan, Kenneth Henry, Michelle L. Twaroski
    Pages 51-65
  6. Pengbo Zhou
    Pages 67-77
  7. Jan Brábek, Steven K. Hanks
    Pages 79-90
  8. Francesca Zappacosta, Michael J. Huddleston, Roland S. Annan
    Pages 91-110
  9. Paul C. Brandt, Thomas C. Vanaman
    Pages 111-127
  10. Hogune Im, Jeffrey A. Grass, Kirby D. Johnson, Meghan E. Boyer, Jing Wu, Emery H. Bresnick
    Pages 129-146
  11. Natasha Elizabeth Zachara, Win Den Cheung, Gerald Warren Hart
    Pages 175-194
  12. Jaeho Lee, Donghang Cheng, Mark T. Bedford
    Pages 195-208
  13. Gil-Soo Han, George M. Carman
    Pages 209-216
  14. Gregory S. Taylor, Jack E. Dixon
    Pages 217-227
  15. Christina C. Leslie, Michael H. Gelb
    Pages 229-242
  16. Hiroko Hama, Javad Torabinejad, Glenn D. Prestwich, Daryll B. DeWald
    Pages 243-258
  17. Sandrine Evellin, Marco Mongillo, Anna Terrin, Valentina Lissandron, Manuela Zaccolo
    Pages 259-270
  18. Chitra Subramanian, Yao Xu, Carl Hirschie Johnson, Albrecht G. von Arnim
    Pages 271-286
  19. Frank van Drogen, Matthias Peter
    Pages 287-306
  20. Nigel J. Waterhouse, Rohan Steel, Ruth Kluck, Joseph A. Trapani
    Pages 307-313
  21. Back Matter
    Pages 315-327

About this book


Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated Signal Transduction Protocols with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and eminently practical, Signal Transduction Protocols, 2nd ed. offers novice and experienced researchers alike an indispensable set of standardized, but readily adaptable, approaches for the assay of key signaling molecules-an invaluable tool for all laboratories newly entering the field, expanding the breadth of previous investigations, or seeking to improve an existing approach.

Editors and affiliations

  • Robert C. Dickson
    • 1
  • Michael D. Mendenhall
    • 1
  1. 1.Department of Molecular and Cellular Biochemistry, College of MedicineUniversity of KentuckyLexington

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-245-2
  • Online ISBN 978-1-59259-816-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site