Checkpoint Controls and Cancer

Volume 2: Activation and Regulation Protocols

  • Axel H. Schönthal

Part of the Methods in Molecular Biology book series (MIMB, volume 281)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Protocols for the Study of Checkpoint Regulatory Components

    1. Front Matter
      Pages 1-1
    2. Christopher N. Mayhew, Emily E. Bosco, David A. Solomon, Erik S. Knudsen, Steven P. Angus
      Pages 3-16
    3. Rati Fotedar, Mourad Bendjennat, Arun Fotedar
      Pages 55-71
    4. Sylke Schneider, Kazumi Uchida, Dennis Salonga, Ji Min Yochim, Kathleen D. Danenberg, Peter V. Danenberg
      Pages 91-103
    5. Axel H. Schönthal
      Pages 105-124
    6. Ya Wang, Hongyan Wang
      Pages 143-151
    7. Ingo Hassepass, Ingrid Hoffmann
      Pages 153-162
    8. Martin F. Lavin, Shaun P. Scott, Sergei Kozlov, Nuri Gueven
      Pages 163-178
    9. Tamotsu Sudo, Naoto T. Ueno, Hideyuki Saya
      Pages 189-198
    10. Paul R. Andreassen, Dimitrios A. Skoufias, Robert L. Margolis
      Pages 213-225
  3. Studying Consequences of Checkpoint Pathway Activation

    1. Front Matter
      Pages 243-243
    2. Heather Beamish, Robyn Warrener, Brian G. Gabrielli
      Pages 245-259
    3. Xiaofen Ye, Maxim Poustovoitov, Hidelita Santos, David M. Nelson, Peter D. Adams
      Pages 261-270
    4. Jill A. Mello, Jonathan G. Moggs, Geneviève Almouzni
      Pages 271-281
    5. Bo Xu, Michael B. Kastan
      Pages 283-292
    6. Piotr Pozarowski, Zbigniew Darzynkiewicz
      Pages 301-311
    7. Stéphanie Plenchette, Rodolphe Filomenko, Emmanuelle Logette, Stéphanie Solier, Nelly Buron, Séverine Cathelin et al.
      Pages 313-331
    8. Katrina E. Gordon, E. Kenneth Parkinson
      Pages 333-348
  4. Back Matter
    Pages 349-358

About this book


Intracellular checkpoint controls constitute a network of signal transduction pathways that protect cells from external stresses and internal errors by means of cell cycle arrest, DNA repair, or apoptosis. Failure of this machinery can lead to embryonic death, genetic diseases, and cancer. In Checkpoint Controls and Cancer, Volume 2: Activation and Regulation Protocols, Axel H. Schönthal, PhD, marshals a prestigious panel of researchers working at the cutting edges of their fields to present readily reproducible experimental protocols for the study of the molecular components of checkpoint controls and their regulation. Described in step-by-step detail, these powerful techniques offer such novel approaches as the use of genome databases and siRNA to analyze how cells of the human body can escape proper surveillance to grow into a tumor. Additional experimental methods are provided for the manipulation of checkpoint pathways and the analysis of the resulting consequences for the cellular phenotype. The earlier first volume, Reviews and Model Systems, comprehensively describes the complexities of checkpoint controls and the model systems available to study them.
Comprehensive and up-to-date, the two volumes of Checkpoint Controls and Cancer offer novice and experienced researchers alike not only entré into the complexities of this vast field, but also to the full panoply of productive tools needed to deepen understanding of the systems, as well as to develop new and more effective therapies.

Editors and affiliations

  • Axel H. Schönthal
    • 1
  1. 1.Department of Molecular Microbiology and Immunology and K. Norris Jr. Comprehensive Cancer CenterUniversity of Southern California Keck School of MedicineLos Angeles

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-500-2
  • Online ISBN 978-1-59259-811-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site