Methods and Protocols

  • Andras Perl

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 102)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Pathogenesis and Spectrum of Autoimmunity

  3. Models of Pathogenesis in Human Autoimmune Disorders

    1. Front Matter
      Pages 9-9
    2. Swapan K. Nath, Jennifer A. Kelly, John B. Harley, R. Hal Scofield
      Pages 11-29
    3. Madhusoodana P. Nambiar, Sandeep Krishnan, George C. Tsokos
      Pages 31-47
    4. Madhusoodana P. Nambiar, Sandeep Krishnan, Vishal G. Warke, George C. Tsokos
      Pages 49-72
    5. Islam U. Khan, Gary M. Kammer
      Pages 73-85
    6. Andras Perl, Gyorgy Nagy, Peter Gergely, Ferenc Puskas, Yueming Qian, Katalin Banki
      Pages 87-114
    7. Kathryn M. Roberts, Antony Rosen, Livia A. Casciola-Rosen
      Pages 115-128
    8. Kyriakos A. Kirou, Christina Lee, Mary K. Crow
      Pages 129-154
  4. Animal Models of Autoimmunity

    1. Front Matter
      Pages 173-173
    2. Daniela Čiháková, Rajni B. Sharma, DeLisa Fairweather, Marina Afanasyeva, Noel R. Rose
      Pages 175-193
    3. Edwin Liu, Liping Yu, Hiroaki Moriyama, George S. Eisenbarth
      Pages 195-212
    4. Martha J. Milton, Michelle Poulin, Clayton Mathews, Jon D. Piganelli
      Pages 213-225
    5. Stanford L. Peng
      Pages 227-272
    6. Bruce Richardson, Donna Ray, Raymond Yung
      Pages 285-294
    7. David D. Brand, Andrew H. Kang, Edward F. Rosloniec
      Pages 295-312
    8. Tibor T. Glant, Katalin Mikecz
      Pages 313-338
    9. Kevin G. Fuller, Julie K. Olson, Laurence M. Howard, J. Ludovic Croxford, Stephen D. Miller
      Pages 339-361
    10. Praveen Rao, Benjamin M. Segal
      Pages 363-375
    11. Gabriella Lakos, Shinsuke Takagawa, John Varga
      Pages 377-393
    12. Rajeev K. Agarwal, Rachel R. Caspi
      Pages 395-419
    13. Arthur A. Hurwitz, Qingyong Ji
      Pages 421-427
  5. Back Matter
    Pages 429-431

About this book


Autoimmunity-which affects up to 10% of the world's population-can damage nearly every tissue or cell type of the human body with greatly debilitating or fatal results. In Autoimmunity: Methods and Protocols, expert medical researchers describe in step-by-step detail molecular techniques and in vitro/in vivo model systems for discovering the causes of autoimmunity, as well as how best it may be regulated. The methods to assess the immunological and biochemical pathways relevant for pathogenesis include identifying susceptibility genes, intercellular signaling via cytokines, intracellular signaling through the T-cell receptor, signal processing via protein kinases, identification and enumeration of antigen-specific T cells and autoantibodies, and the dysregulation of apoptosis and its role in modification of self-antigens. Additional protocols serve to establish the presence of and assess inflammatory arthritis, systemic lupus erythematosus, insulin-dependent diabetes mellitus, multiple sclerosis, myocarditis, thyroiditis, scleroderma, uveitis, and vitiligo. These methods can be used to assess the genetic, immunological, and biochemical parameters underlying spontaneous or exogenous antigen-induced diseases. The protocols follow the successful Methods in Molecular Medicine™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Innovative and easy to follow, Autoimmunity: Methods and Protocols offers today's investigators a cutting-edge collection of readily reproducible methods for establishing and interrogating human and animal models of autoimmune diseases.

Editors and affiliations

  • Andras Perl
    • 1
  1. 1.Departments of Medicine, Microbiology, and ImmunologyState University of New York, Upstate Medical University, College of MedicineSyracuse

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-231-5
  • Online ISBN 978-1-59259-805-2
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site