Optimization in Drug Discovery

In Vitro Methods

  • Zhengyin Yan
  • Gary W. Caldwell

Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Yushen Guo, Hong Shen
    Pages 1-17
  3. Ming Hu, Jie Ling, Huimin Lin, Jun Chen
    Pages 19-35
  4. Jeffrey A. Ruell, Alex Avdeef
    Pages 37-64
  5. Eun Ju Jeong, Yan Liu, Huimin Lin, Ming Hu
    Pages 65-76
  6. Seong-Hee Park, Sung-Hack Lee, Yaming Su, Patrick J. Sinko
    Pages 77-87
  7. Lucinda H. Cohen
    Pages 111-122
  8. David C. Ackley, Kevin T. Rockich, Timothy R. Baker
    Pages 151-162
  9. Daniel R. Mudra, Andrew Parkinson
    Pages 203-214
  10. Zhengyin Yan, Gary W. Caldwell
    Pages 231-244
  11. Amin A. Nomeir, Jairam R. Palamanda, Leonard Favreau
    Pages 245-262
  12. Naomi Suzuki, Padmaja M. Prabhu, Shinya Shibutani
    Pages 263-278
  13. Bojana Žegura, Metka Filipič
    Pages 301-313
  14. Xuming Jia, Wei Xiao
    Pages 315-323
  15. Atsushi Hakura, Satoshi Suzuki, Tetsuo Satoh
    Pages 325-336
  16. Tao Chen, Martha M. Moore
    Pages 337-352
  17. Keith Finlayson, John Sharkey
    Pages 353-368
  18. Wei Tang, Randy R. Miller
    Pages 369-383
  19. Back Matter
    Pages 405-418

About this book


Although parallel synthesis combined with high-throughput screening has made it easier to generate highly potent drug candidates, many of these compounds never make the grade because they either prove to be unsafe or lack the necessary physicochemical and pharmacokinetic properties. In Optimization in Drug Discovery: In Vitro Methods, a panel of researchers and experts from leading universities and major pharmaceutical companies from all over the world provide proven solutions to this expensive problem with a collection of cutting-edge experimental protocols for early in vitro evaluation of new chemical entities (NCE). These readily reproducible assays measure such critical parameters as physicochemical properties, adsorption, plasma binding, metabolism, drug interactions, and toxicity. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Optimization in Drug Discovery: In Vitro Methods systematically collects and describes the key methods for uncovering-as early in the discovery stage as possible-those defects that can eliminate candidate drugs from preclinical and clinical development.

Editors and affiliations

  • Zhengyin Yan
    • 1
  • Gary W. Caldwell
    • 1
  1. 1.Johnson & Johnson Pharmaceutical Research & DevelopmentSpring House

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-332-9
  • Online ISBN 978-1-59259-800-7
  • Series Print ISSN 1557-2153
  • Series Online ISSN 1940-6053
  • Buy this book on publisher's site