Parasite Genomics Protocols

  • Sara E. Melville

Part of the Methods in Molecular Biology™ book series (MIMB, volume 270)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Daniella Bartholomeu, Najib M. El-Sayed
    Pages 1-16
  3. Matthew Berriman, Midori Harris
    Pages 17-43
  4. Christiane Hertz-Fowler, Neil Hall
    Pages 45-74
  5. Claire Whitton, Jennifer Daub, Marian Thompson, Mark Blaxter
    Pages 75-91
  6. John Parkinson, Mark Blaxter
    Pages 93-126
  7. Winston A. Hide, Raphael D. Isokpehi
    Pages 127-150
  8. Bryony A. P. Williams, Robert P. Hirt
    Pages 151-171
  9. Daniel K. Masiga, C. Michael R. Turner
    Pages 173-185
  10. Karl F. Hoffmann, Jennifer M. Fitzpatrick
    Pages 219-236
  11. Upinder Singh, Preetam H. Shah, Ryan C. MacFarlane
    Pages 237-248
  12. Chunlei Su, Christian Hott, Bernard H. Brownstein, L. David Sibley
    Pages 249-262
  13. Brendan S. Crabb, Melanie Rug, Tim-Wolf Gilberger, Jennifer K. Thompson, Tony Triglia, Alexander G. Maier et al.
    Pages 263-276
  14. George K. Arhin, Shuiyuan Shen, Elisabetta Ullu, Christian Tschudi
    Pages 277-286
  15. Appolinaire Djikeng, Shuiyuan Shen, Christian Tschudi, Elisabetta Ullu
    Pages 287-297
  16. Kelly A. Robinson, Sophie Goyard, Stephen M. Beverley
    Pages 299-317
  17. Vanessa Leech, Michael A. Quail, Sara E. Melville
    Pages 335-351
  18. Pascal Dubessay, Christine Blaineau, Patrick Bastien, Michel Pagès
    Pages 353-377
  19. Hirohisa Hirai, Yuriko Hirai
    Pages 379-394
  20. Carmel B. Stober
    Pages 423-437
  21. Back Matter
    Pages 439-452

About this book


Parasitic disease remains an important health issue in most regions of the world and constitutes a major cause of morbidity and mortality in developing countries. In Parasite Genomics Protocols, pioneering bench researchers describe in detail the cutting-edge techniques they have developed for analyzing the genomes and gene products in a diverse range of protozoan and metazoan parasites. These readily reproducible techniques can be used in genomic, functional genomic, and postgenomic studies and include transfection methods and vectors for several protozoan parasites, global analysis using microarrays, gene ablation using RNA interference, gene knockout, mutagenesis, and chromosome manipulation. Some of the protocols require DNA sequence data, whereas others were developed independently of whole-genome sequence data. Additional chapters survey the available genome sequences, helping novice researchers overcome the difficulties of accessing the databases and applying bioinformatics analysis to biological function. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and the avoidance of known pitfalls.
State-of-the-art and highly practical, Parasite Genomics Protocols offers bench investigators in parasitology a wide-ranging collection of diverse methods for elucidating the content of the genomes of parasitic organisms, utilizing the genome sequence for genetic analysis, and ascribing function to the available genome sequences.

Editors and affiliations

  • Sara E. Melville
    • 1
  1. 1.Department of PathologyUniversity of CambridgeCambridgeUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-062-5
  • Online ISBN 978-1-59259-793-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site