Platelets and Megakaryocytes

Volume 1: Functional Assays

  • Jonathan M. Gibbins
  • Martyn P. Mahaut-Smith

Part of the Methods In Molecular Biology™ book series (MIMB, volume 272)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Platelet Functional Assays

    1. Front Matter
      Pages 1-1
    2. Jean-Pierre Cazenave, Philippe Ohlmann, Dominique Cassel, Anita Eckly, Béatrice Hechler, Christian Gachet
      Pages 13-28
    3. Paul Harrison, Carol Briggs, Samuel J. Machin
      Pages 29-46
    4. Gavin E. Jarvis
      Pages 65-76
    5. Gavin E. Jarvis
      Pages 77-87
    6. M. Fred Heath
      Pages 89-93
    7. David Crosby, Alastair W. Poole
      Pages 95-96
    8. Joachim Jankowski, Lars Henning, Hartmut Schlüter
      Pages 97-108
    9. Tara W. Rutledge, Sidney W. Whiteheart
      Pages 109-120
    10. Richard W. Farndale, Philip G. Hargreaves, Joanna L. Dietrich, Rosemary J. Keogh
      Pages 121-133
    11. Johan W. M. Heemskerk, Paul Comfurius, Marion A. H. Feijge, Edouard M. Bevers
      Pages 135-144
    12. Renata Polanowska-Grabowska, Adrian R. L. Gear
      Pages 153-163
    13. Suhasini Kulkarni, Warwick S. Nesbitt, Sacha M. Dopheide, Sascha C. Hughan, Ian S. Harper, Shaun P. Jackson
      Pages 165-186
    14. Shahrokh Falati, Peter L. Gross, Glenn Merrill-Skoloff, Derek Sim, Robert Flaumenhaft, Alessandro Celi et al.
      Pages 187-197
    15. Bernhard Nieswandt, Valerie Schulte, Wolfgang Bergmeier
      Pages 255-268
    16. Shosaku Nomura, Shirou Fukuhara
      Pages 269-277
  3. Megakaryocyte Functional Assays

    1. Front Matter
      Pages 279-279
    2. Najet Debili, Fawzia Louache, William Vainchenker
      Pages 293-308
    3. Anthony Mathur, Ying Hong, Guosu Wang, Jorge D. Erusalimsky
      Pages 309-322
    4. Arnaud Drouin, Gulie Alimardani, Elisabeth M. Cramer
      Pages 323-345
    5. Warren S. Alexander, Craig Hyland
      Pages 347-359
    6. Norio Komatsu
      Pages 361-373
  4. Back Matter
    Pages 375-385

About this book


Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 1: Functional Assays, leading expert researchers-many responsible for seminal discoveries in the field-describe basic and advanced techniques for analyzing platelet and megakaryocyte function in step-by-step detail. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. Techniques presented for megakaryocyte function research include those used to study the development of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques offers a state-of-the-art survey of our understanding of these cells, with emphasis on the role of cell signaling in their regulation and function, as well as the use of molecular and post-genomic techniques for their study.
Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

Editors and affiliations

  • Jonathan M. Gibbins
    • 1
  • Martyn P. Mahaut-Smith
    • 2
  1. 1.School of Animal and Microbial SciencesThe University of ReadingReadingUK
  2. 2.Department of PhysiologyUniversity of CambridgeCambridgeUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-101-1
  • Online ISBN 978-1-59259-782-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site