Tumor Necrosis Factor

Methods and Protocols

  • Angelo Corti
  • Pietro Ghezzi

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 98)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Pietro Ghezzi, Anthony Cerami
    Pages 1-8
  3. Hartmut Engelmann, Dani Aderka, David Wallach
    Pages 23-31
  4. Paul Ameloot, Peter Brouckaert
    Pages 33-45
  5. Silvia Fargion, Luca Valenti, Paola Dongiovanni, Anna Ludovica Fracanzani
    Pages 47-58
  6. Roberto Furlan, Giuliana Salani, Alessandra Bergami, Gianvito Martino
    Pages 59-72
  7. Marta Muzio, Simona Saccani
    Pages 81-99
  8. Tom Vanden Berghe, Geertrui Denecker, Greet Brouckaert, Dmitri Vadimovisch Krysko, Katharina D’Herde, Peter Vandenabeele
    Pages 101-126
  9. Eleni Douni, Maria Alexiou, George Kollias
    Pages 137-169
  10. Roberto Furlan, Pia Villa, Giorgio Senaldi, Gianvito Martino
    Pages 171-190
  11. Maria Elena Ferrero
    Pages 191-198
  12. Pia Villa, Pietro Ghezzi
    Pages 199-206
  13. Stefano Chimenti, Eleonora Carlo, Serge Masson, Antonio Bai, Roberto Latini
    Pages 217-225
  14. Timo L. M. ten Hagen, Alexander M. M. Eggermont
    Pages 227-245
  15. Back Matter
    Pages 265-270

About this book


Because tumor necrosis factor-a (TNF-a) plays a pivotal role in the regulation of homeostasis and inflammatory immune responses, it offers valuable research opportunities to develop new drugs for the treatment of a wide range of disorders, including cancer, septic shock, rheumatoid arthritis, and other inflammatory diseases. In Tumor Necrosis Factor: Methods and Protocols, well-versed experimentalists survey the basic and translational research being conducted in this field and describe in detail the methods they have developed for TNF production, characterization, mutagenesis, and detection in biological specimens. They also provide several in vitro assays and animal models for studying the role of TNF in various TNF-related diseases and in cancer. The protocols presented follow the successful Methods in Molecular Medicine™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Tumor Necrosis Factor: Methods and Protocols offers molecular and cellular biologists, pharmacologists, and toxicologists a diverse set of productive, cutting-edge tools for illuminating the pathophysiological roles of TNF in disease and for identifying new drugs.

Editors and affiliations

  • Angelo Corti
    • 1
  • Pietro Ghezzi
    • 2
  1. 1.Department of Biological and Technological Research, Cancer Immunotherapy and Gene Therapy ProgramSan Raffaele H Scientific InstituteMilanItaly
  2. 2.Mario Negri Institute for Pharmacological ResearchMilanItaly

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-223-0
  • Online ISBN 978-1-59259-771-0
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site