Public Health Microbiology

Methods and Protocols

  • John F. T. Spencer
  • Alicia L. Ragout de Spencer

Part of the Methods in Molecular Biology book series (MIMB, volume 268)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Bacteria

    1. Front Matter
      Pages 1-1
    2. Rustam I. Aminov, Joanne C. Chee-Sanford, Natalie Garrigues, Asma Mehboob, Roderick I. Mackie
      Pages 3-13
    3. Brigid Lucey, Fiona O’Halloran, Séamus Fanning
      Pages 33-47
    4. Ana Belén Vivanco, Juan Alvarez, Idoia Laconcha, Nuria López-Molina, Aitor Rementeria, Javier Garaizar
      Pages 49-57
  3. Viruses

    1. Front Matter
      Pages 59-59
    2. Albert Bosch, Rosa M. Pintó, Jaume Comas, Francesc-Xavier Abad
      Pages 61-68
    3. Fiona O’Halloran, Séamus Fanning
      Pages 89-102
    4. Glòria Sánchez, Cristina Villena, Albert Bosch, Rosa M. Pintó
      Pages 103-114
  4. Fungi

    1. Front Matter
      Pages 115-115
    2. Aitor Rementeria, Ana Belén Vivanco, Ainara Cadaval, María Teresa Ruesga, Sonia Brena, José Pontón et al.
      Pages 117-125
    3. Dante Javier Bueno, Julio Oscar Silva, Guillermo Oliver
      Pages 127-131
    4. Dante Javier Bueno, Guillermo Oliver
      Pages 133-137
  5. Other Pathogens

    1. Front Matter
      Pages 139-139
    2. Judith A. Noble-Wang, Shangtong Zhang, Daniel Price, Donald G. Ahearn
      Pages 153-161
    3. Lihua Xiao, Altaf A. Lal, Jianlin Jiang
      Pages 163-176
    4. Giovanni Widmer, Xiaochuan Feng, Sultan Tanriverdi
      Pages 177-187
    5. Gary P. Yakub, Kathleen L. Stadterman-Knauer
      Pages 189-197
    6. Diana Draksler, María Cecilia Monferran, Silvia González
      Pages 207-211
  6. Bacteriocins and Other Inhibitors

    1. Front Matter
      Pages 223-223
    2. Jean-Marc Berjeaud, Yves Cenatiempo
      Pages 225-233
    3. Anavella Gaitan Herrera
      Pages 281-288
    4. Anavella Gaitan Herrera
      Pages 289-291
    5. Anavella Gaitan Herrera
      Pages 293-295
    6. Anavella Gaitan Herrera
      Pages 297-301
    7. Tânia L. P. Pessatti, José D. Fontana, Marcos L. Pessatti
      Pages 311-316
    8. María L. Tereschuk, Mario D. Baigorí, Lucia I. C. de Figueroa, Lidia R. Abdala
      Pages 317-330
    9. Lucila Saavedra, Patricia Castellano, Fernando Sesma
      Pages 331-336
    10. María Silvina Juárez Tomás, María Claudia Otero, Virginia Ocaña, María Elena Nader-Macías
      Pages 337-346
    11. Virginia S. Ocaña, María Elena Nader-Macías
      Pages 347-353
    12. María Silvina Juárez Tomás, Elena Bru, María Elena Nader-Macías
      Pages 355-365
    13. Graciela Vignolo, Patricia Castellano
      Pages 367-370
  7. In Vivo Studies in Mice

    1. Front Matter
      Pages 371-371
    2. Rosa Cangemi de Gutierrez, Viviana M. Santos, María Elena Nader-Macías
      Pages 373-385
    3. Elisa Vintiñi, Virginia Ocaña, María Elena Nader-Macías
      Pages 401-410
    4. Carlos Gusils, Vilma Morata, Silvia González
      Pages 411-415
    5. María Pía Taranto, Gabriela Perdigón, Marta Médici, Graciela Font de Valdez
      Pages 417-422
  8. Special Methods

    1. Front Matter
      Pages 433-433
    2. María Claudia Otero, Virginia S. Ocaña, María Elena Nader-Macías
      Pages 435-440
    3. Virginia S. Ocaña, María Elena Nader-Macías
      Pages 441-445

About this book


The world abounds with microorganisms that are hazardous to human health in the normal course of life. In Public Health Microbiology: Methods and Protocols, expert investigators describe in step-by-step detail the laboratory techniques they have perfected for determining the nature and character of a diverse group of hazardous bacteria, viruses, and fungi. For bacterial and viral pathogens, the authors give special attention to PCR methods for detecting genes resistant to tetracycline, for resistance in Salmonella enterica, for identifying and typing Campylobacter coli, and for detecting the abundance of enteric, hepatitis A, and rotaviruses in sewage, as well as bacteriophages infecting the O157:H7 strain of Escherichia coli. For fungi the authors offer methods for computerized analysis and typing of fungal isolates, for the isolation and enumeration of fungi in foods, and for the determination of aflatoxin and zearalenone. Other methods deal with hazard analysis, the use of disinfectants, microbiological analysis of cosmetics, microbiological tests for sanitation equipment in factories, and the uses of animals in studies of various characteristics of lactobacilli. Review articles address the spread of pathogens from livestock and poultry production, including the threat of prion-based diseases. All protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Timely and highly practical, Public Health Microbiology: Methods and Protocols offers researchers and public health specialists alike a wide-ranging collection of methods for the determination of many important pathogenic microorganisms, their effects on health, and possible measures that can be taken to counter these effects.

Editors and affiliations

  • John F. T. Spencer
    • 1
  • Alicia L. Ragout de Spencer
    • 1
  1. 1.Planta Piloto de Procesos Industriales Microbiológicos(PROIMI)-CONICETSan Miguel de TucumánArgentina

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-117-2
  • Online ISBN 978-1-59259-766-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site