Environmental Microbiology

Methods and Protocols

  • John M. Walker
  • John F. T. Spencer
  • Alicia L. Ragout de Spencer

Part of the Methods in Biotechnology book series (MIBT, volume 16)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Communities and Biofilms

    1. Front Matter
      Pages 1-1
    2. Juliana M. Benito, Gustavo A. Lovrich, Faustino Siñeriz, Carlos M. Abate
      Pages 3-9
    3. E. Anne Greene, Gerrit Voordouw
      Pages 11-22
    4. Werner Liesack, Peter F. Dunfield
      Pages 23-37
    5. Garrett W. Pernèy, Betsy R. Robertson, D. K. Button
      Pages 39-50
    6. Nicolás G. Barengo, Juliana M. Benito, Carlos M. Abate
      Pages 75-82
    7. Lucía I. C. de Figueroa, M. Alejandra Martínez, John F. T. Spencer
      Pages 83-96
    8. Anavella Gaitan Herrera
      Pages 97-102
    9. Eric S. Gilbert, Jay D. Keasling
      Pages 109-118
  3. Fermented Milks

    1. Front Matter
      Pages 119-119
    2. Marisa S. Garro, Graciela Font de Valdez, Graciela Savoy de Giori
      Pages 121-124
    3. Marisa S. Garro, Graciela Font de Valdez, Graciela Savoy de Giori
      Pages 135-138
    4. Elvira M. Hébert, Raúl R. Raya, Graciela Savoy de Giori
      Pages 139-148
  4. Nuclei Acids, Recovery, and Determination

    1. Front Matter
      Pages 149-149
    2. Raúl O. Pedraza, Juan C. Díaz Ricci
      Pages 151-157
    3. Anne-Marie Delort, Genevieve Gaudet, Evelyne Forano
      Pages 173-188
    4. Ana Gutiérrez, José C. del Río, Ángel T. Martínez
      Pages 189-202
    5. José Domingos Fontana, Juliana Adelmann, Mauricio Passos, Marcelo Maraschin, Cristina A. de Lacerda, Fernando Mauro Lanças
      Pages 203-218
    6. Luiz Pereira Ramos, José Domingos Fontana
      Pages 219-233
    7. Kazuya Watanabe, Natsuko Hamamura, Nobuo Kaku
      Pages 235-242
    8. Virginia H. Albarracín, Juliana M. Benito, Manuel Siñeriz Louis, María J. Amoroso, Carlos M. Abate
      Pages 243-247
    9. Liliana Villegas, María J. Amoroso, Lucía I. C. de Figueroa
      Pages 249-256
    10. Mónica M. Locascio, Rosalía Alesso, Vilma I. Morata, Silvia N. González
      Pages 257-261
    11. M. Carina Audisio, María C. Apella
      Pages 263-267
    12. Fabiana M. Saguir, María C. Manca de Nadra
      Pages 269-273
    13. Claudia S. Benimeli, Adriana P. Chaile, María J. Amoroso
      Pages 279-282
    14. Fabio Vazquez, Martha Dina Vallejo Herrera, Lucía I. C. de Figueroa, María Eugenia Toro
      Pages 283-297
    15. Martha Dina Vallejo Herrera, María Eugenia Toro, Lucía I. C. de Figueroa, Fabio Vazquez
      Pages 299-321
    16. Carina Van Nieuwenhove, Silvia N. González, Alicia Bardón
      Pages 353-356
  5. Reviews

    1. Front Matter
      Pages 357-357
    2. Charles W. Bacon, Dorothy M. Hinton
      Pages 359-377
    3. Anne-Marie Delort, Genevieve Gaudet, Evelyne Forano
      Pages 389-405
  6. Back Matter
    Pages 419-423

About this book


Understanding the relationship between a microorganism and its environment is essential to the successful manipulation of industrial, biochemical, and medical processes. In Environmental Microbiology: Methods and Protocols, highly practiced experimentalists who often have perfected the methods they write about describe readily reproducible techniques for determining most of the important factors governing microorganisms and their habitats. Presented in step-by-step detail, these cutting-edge methods range from those for the study of marine organisms, to those for investigating microorganisms occurring in groundwater, to the biodiversity found in remote environments. The protocols for studying fermented milks are significant for investigators concerned with milk as an item of food for infants, small children, and even adults. Additional methods for the recovery and determination of nucleic acids and other compounds affecting, and affected by, microorganisms, are provided for certain enzymes produced by plant pathogens and for obtaining microbial species tolerant of such inhibitors as heavy metals. Review articles discuss the endophytic bacterium Bacillus mojavensis, the engineering of bacteria to enhance their ability to carry out bioremediation of aromatic compounds, and the use of chemical shift reagents and Na-NMR to study sodium gradients in microorganisms. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Environmental Microbiology: Methods and Protocols offers microbiological researchers a powerful set of techniques for investigating and understanding microorganisms in their native environments.

Editors and affiliations

  • John M. Walker
  • John F. T. Spencer
    • 1
  • Alicia L. Ragout de Spencer
    • 1
  1. 1.Planta Piloto de Procesos Industriales Microbiológicos (PROIMI)-CONICETSan Miguel de TucumánArgentina

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-116-5
  • Online ISBN 978-1-59259-765-9
  • Series Print ISSN 1940-6061
  • Series Online ISSN 1940-607X
  • Buy this book on publisher's site