Protein-Protein Interactions

Methods and Applications

  • Haian Fu

Part of the Methods in Molecular Biology book series (MIMB, volume 261)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Overview

    1. Front Matter
      Pages 1-1
    2. Robert C. Liddington
      Pages 3-14
  3. In Vitro Techniques

    1. Front Matter
      Pages 33-33
    2. Adrian Velazquez-Campoy, Stephanie A. Leavitt, Ernesto Freire
      Pages 35-54
    3. Guanghua Gao, Jason G. Williams, Sharon L. Campbell
      Pages 79-91
    4. Ian A. Taylor, John F. Eccleston, Katrin Rittinger
      Pages 119-136
    5. Rosemarie Wilton, Elizabeth A. Myatt, Fred J. Stevens
      Pages 137-154
    6. Sang-Hyun Park, Ronald T. Raines
      Pages 155-159
    7. Sang-Hyun Park, Ronald T. Raines
      Pages 161-165
  4. Detecting Protein-Protein Interactions in Heterologous Systems

    1. Front Matter
      Pages 229-229
    2. Simon L. Dove, Ann Hochschild
      Pages 231-246
    3. Safia Thaminy, John Miller, Igor Stagljar
      Pages 297-312
    4. Matthew A. Bennett, Jack F. Shern, Richard A. Kahn
      Pages 313-326
    5. Shane C. Masters
      Pages 337-348
  5. Probing Protein-Protein Interactions in Living Cells

    1. Front Matter
      Pages 349-349
    2. Brian Herman, R. Venkata Krishnan, Victoria E. Centonze
      Pages 351-370
    3. Keith M. Berland
      Pages 383-397
    4. Ingrid Remy, Stephen W. Michnick
      Pages 411-426
    5. Fabrice Agou, Fei Ye, Michel Véron
      Pages 427-442
  6. Proteomics-Based Approaches

    1. Front Matter
      Pages 443-443
    2. John C. Obenauer, Michael B. Yaffe
      Pages 445-467
    3. Greg Moorhead, Carol MacKintosh
      Pages 469-477
    4. Huilin Zhou, Rosemary Boyle, Ruedi Aebersold
      Pages 511-518
  7. Back Matter
    Pages 525-532

About this book


Proteins continuously interact with each other to determine cell fate. Consequently, an examination of just when such protein-protein interactions occur and how they are controlled is essential for understanding the molecular mechanism of biological processes, elucidating the molecular basis of diseases, and identifying potential targets for therapeutic interventions. In Protein-Protein Interactions: Methods and Applications, leading experts describe in detail their highly successful biochemical, biophysical, genetic, and computational techniques for studying these interactions. Their readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics- and bioinformatics-based approaches, such as the Scansite program for computational analysis. Each time-tested protocol includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Protein-Protein Interactions: Methods and Applications offers both beginning and experienced investigators a full range of the powerful tools needed for deciphering how proteins interact to form biological networks, as well as for unraveling protein-protein interactions in disease in the search for novel therapeutic targets.

Editors and affiliations

  • Haian Fu
    • 1
  1. 1.Department of PharmacologyEmory University School of MedicineAtlanta

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-120-2
  • Online ISBN 978-1-59259-762-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site