Mobile Genetic Elements

Protocols and Genomic Applications

  • Wolfgang J. Miller
  • Pierre Capy

Part of the Methods in Molecular Biology book series (MIMB, volume 260)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Wolfgang J. Miller, Pierre Capy
    Pages 1-20
  3. Christian Biémont, Laurence Monti-Dedieu, FranÇoise Lemeunier
    Pages 21-28
  4. I. King. Jordan, Nathan J. Bowen
    Pages 59-71
  5. Andreas Bachmair, Karin Garber, Shin Takeda, Kazuhiko Sugimoto, Tetsuji Kakutani, Hirohiko Hirochika
    Pages 73-82
  6. William S. Reznikoff, Igor Y. Goryshin, Jerry J. Jendrisak
    Pages 83-96
  7. Gregory B. Gloor
    Pages 97-114
  8. Michiel Vandenbussche, Tom Gerats
    Pages 115-127
  9. Alan H. Schulman, Andrew J. Flavell, T. H. Noel Ellis
    Pages 145-173
  10. Alexandra M. Casa, Alexander Nagel, Susan R. Wessler
    Pages 175-188
  11. Norihiro Okada, Andrew M. Shedlock, Masato Nikaido
    Pages 189-226
  12. David A. O’ Brochta, Peter W. Atkinson
    Pages 227-254
  13. Back Matter
    Pages 277-289

About this book


Transposable elements (TEs)-DNA sequences that are capable of moving from one chromosome location to another-are found in all living organisms. They have been increasingly investigated in a wide spectrum of species, including bacteria, plants, fungi, and animals. In Mobile Genetic Elements: Protocols and Genomic Applications, leading experts describe in step-by-step detail their most productive transposon-based methods and strategies for studying genome structure, function, and evolution. These readily reproducible techniques cover a broad range, including mutagenesis, transgenesis, gene silencing, and molecular systematics. Among the highlights are a series of DNA hybridization methods for analyzing the distribution and dynamics of mobile DNA at the hosts' genomic level, techniques for studying LTR retrotransposons in heterologous host systems, and mutagenesis protocols for investigating gene functions in a broad range of organisms. Additional methods deal with highly informative sets of polymorphic markers, RNAi technology in gene silencing, and applications during transgenesis. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Mobile Genetic Elements: Protocols and Genomic Applications offers investigators powerful genetic tools for dissecting the function of a specific gene, elaborating on the mechanisms leading to genetic change and diversity, and studying the evolutionary impact of mobile DNA on the biology and evolution of organisms.

Editors and affiliations

  • Wolfgang J. Miller
    • 1
  • Pierre Capy
    • 2
  1. 1.AG General Genetics, Institute of Medical BiologyUniversity of ViennaViennaAustria
  2. 2.Laboratoire Populations, Génétique et EvolutionCNRSGif-sur-YvetteFrance

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-007-6
  • Online ISBN 978-1-59259-755-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site