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Bacterial Artificial Chromosomes

Volume 1: Library Construction, Physical Mapping, and Sequencing

Part of the book series: Methods in Molecular Biology (MIMB, volume 255)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xx
  2. BAC Library Construction

    • Kazutoyo Osoegawa, Pieter J. de Jong
    Pages 1-46
  3. Construction of Small Genome BAC Library for Functional and Genomic Applications

    • David Å majs, Steven J. Norris, George M. Weinstock
    Pages 47-56
  4. Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging

    • Sangita Pal, Solida Mak, George M. Weinstock
    Pages 57-68
  5. Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions

    • Natalay Kouprina, Vladimir N. Noskov, Maxim Koriabine, Sun-Hee Leem, Vladimir Larionov
    Pages 69-89
  6. Purification of BAC DNA

    • Tim S. Poulsen
    Pages 91-100
  7. Hybridization-Based Selection of BAC Clones

    • Chang-Su Lim, Ung-Jin Kim
    Pages 101-111
  8. Applications of Interspersed Repeat Sequence Polymerase Chain Reaction

    • Heike Zimdahl, Claudia Gösele, Thomas Kreitler, Margit Knoblauch
    Pages 113-129
  9. BAC Mapping Using Fluorescence In Situ Hybridization

    • Xiao-Ning Chen, Julie R. Korenberg
    Pages 131-141
  10. High-Throughput BAC Fingerprinting

    • Jacqueline Schein, Tamara Kucaba, Mandeep Sekhon, Duane Smailus, Robert Waterston, Marco Marra
    Pages 143-156
  11. BAC End Sequencing

    • Tim S. Poulsen, Hans E. Johnsen
    Pages 157-161
  12. Radiation Hybrid Mapping With BAC Ends

    • Michael Olivier, Shannon Brady, David R. Cox
    Pages 163-169
  13. Rolling Circle Amplification for Sequencing Templates

    • Paul F. Predki, Chris Elkin, Hitesh Kapur, Jamie Jett, Susan Lucas, Tijana Glavina et al.
    Pages 189-196
  14. Transposon-Mediated Sequencing

    • Rachel Reeg, Anup Madan
    Pages 197-210
  15. Pyrosequencing

    • Elahe Elahi, Mostafa Ronaghi
    Pages 211-219
  16. BAC Finishing Strategies

    • Christine Bird, Darren Grafham
    Pages 255-277

About this book

For both volumes:

Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.

Editors and Affiliations

  • The Institute for Genomic Research, Rockville

    Shaying Zhao

  • Office of Biological and Environmental Research, US Department of Energy, Germantown

    Marvin Stodolsky

Bibliographic Information

  • Book Title: Bacterial Artificial Chromosomes

  • Book Subtitle: Volume 1: Library Construction, Physical Mapping, and Sequencing

  • Editors: Shaying Zhao, Marvin Stodolsky

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597521

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-0-89603-988-9Published: 04 March 2004

  • Softcover ISBN: 978-1-61737-288-9Published: 10 November 2010

  • eBook ISBN: 978-1-59259-752-9Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XX, 360

  • Number of Illustrations: 71 b/w illustrations

  • Topics: Bacteriology, Microbiology, Biochemistry, general

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access