Adrenergic Receptor Protocols

  • Curtis A. Machida

Part of the Methods in Molecular Biology™ book series (MIMB, volume 126)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Genetic Analysis

    1. Front Matter
      Pages 1-1
    2. Margaret A. Scofield, Jean D. Deupree, David B. Bylund
      Pages 3-35
    3. Margaret A. Scofield, Jean D. Deupree, David B. Bylund
      Pages 37-51
    4. Jean D. Deupree, Margaret A. Scofield, David B. Bylund
      Pages 53-72
    5. Ian P. Hall, Amanda P. Wheatley, Jane C. Dewar
      Pages 117-126
  3. RNA Analysis

  4. Expression Analysis

    1. Front Matter
      Pages 187-187
    2. Stéphane Schaak, Jean-Christophe Devedjian, Hervé Paris
      Pages 189-206
    3. Ralf Jockers, Margaret Scofield
      Pages 215-220
    4. Yi-Tang Tseng, James F. Padbury
      Pages 235-239
    5. Hsien-Yu Wang, Fubao Lin, Craig C. Malbon
      Pages 241-258
    6. Daniel K. Rohrer
      Pages 259-277
  5. Receptor and Second Messenger Analysis

    1. Front Matter
      Pages 279-279
    2. Suleiman W. Bahouth
      Pages 281-299
    3. Suleiman W. Bahouth
      Pages 301-314
    4. Michael K. Sievert, John F. Resek, Zhongren Wu, Yajing Rong, Arnold E. Ruoho
      Pages 315-327
    5. Cheryl D. Dunigan, Patricia K. Curran, Peter H. Fishman
      Pages 329-343
    6. Steven R. Post, Rennolds S. Ostrom, Paul A. Insel
      Pages 363-374
  6. Transactivator Analysis

  7. Microscopy Analysis

  8. Back Matter
    Pages 565-577

About this book


In Adrenegic Receptor Protocols, Curtis Machida and a panel of expert investigators present a comprehensive collection of modern molecular methods for analyzing adrenergic receptors and corresponding second messenger systems. These proven and readily reproducible techniques utilize genetic, RNA, protein expression, transactivator, and second messenger methodologies, as well as immunocytochemical, electrophysiological, transgenic, and in situ hybridization approaches. Each of the experts writing here details the use of their chosen method in examining the adrenergic receptor system, using aspects of the genetic flow of information as a guide: DNA ' RNA ' transactivator ' protein expression ' second messenger analyses ' cellular analyses ' transgenic whole animal approaches.
Comprehensive and rich in practical detail, Adrenergic Receptor Protocols provides the first collection of reproducible methods for the study of these important regulators of CNS-mediated behavior and neural function. Its state-of-the-art methods constitute today's gold standard reference for all neurobiologists, neurochemists, neurologists, and pharmacologists studying this exceptionally important class of receptors.

Editors and affiliations

  • Curtis A. Machida
    • 1
  1. 1.Oregon Regional Primate Research CenterOregon Health Sciences UniversityBeaverton

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-602-4
  • Online ISBN 978-1-59259-684-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site