Molecular Diagnosis of Infectious Diseases

  • Jochen Decler
  • Udo Reischl

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 94)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Protein-Based Identification Of Antigens

    1. Front Matter
      Pages 1-1
    2. Karen M. Dobos, John S. Spencer, Ian M. Orme, John T. Belisle
      Pages 3-17
    3. Alexander Krah, Peter R. Jungblut
      Pages 19-32
    4. Stephen Thompson
      Pages 33-45
  3. DNA-Based Identification of Antigens

    1. Front Matter
      Pages 47-47
    2. Lucas D. Bowler
      Pages 49-66
    3. Silvia Saviozzi, Giovanni Iazzetti, Enrico Caserta, Alessandro Guffanti, Raffaele A. Calogero
      Pages 67-90
    4. Michael J. Lodes, Davin C. Dillon, Raymond L. Houghton, Yasir A. W. Skeiky
      Pages 91-106
  4. Identification of Epitopes and Immunomodulatory Components

    1. Front Matter
      Pages 107-107
    2. Christine Hager-Braun, Kenneth B. Tomer
      Pages 109-120
    3. Timothy W. Tobery, Michael J. Caulfield
      Pages 121-132
    4. Ludwig Deml, Jens Wild, Ralf Wagner
      Pages 133-157
    5. Ludwig Deml, Michael Aigner, Alexander Eckhardt, Jochen Decker, Norbert Lehn, Wulf Schneider-Brachert
      Pages 159-175
  5. Expression of Recombinant Proteins

  6. Purification, Modification, and Renaturation of Recombinant Proteins

  7. Characterization of Recombinant Proteins

    1. Front Matter
      Pages 267-267
    2. Claudia Hahnefeld, Stephan Drewianka, Friedrich W. Herberg
      Pages 299-320
    3. Uwe Schaible, Michael Liss, Elke Prohaska, Jochen Decker, Karin Stadtherr, Hans Wolf
      Pages 321-330
  8. Evaluation of Recombinant Proteins in Immunological Test Systems

  9. Recombinant Receptor Molecules

    1. Front Matter
      Pages 445-445
    2. Lars Toleikis, Olaf Broders, Stefan Dübel
      Pages 447-458
  10. Back Matter
    Pages 459-464

About this book


In this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors demonstrate the power of these new methodologies in both established and novel procedures by applying them successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, and their use in diagnostic reagents in immunological assays. Basic problems of serological laboratory diagnostics and the use of immunoproteomics for discovering target proteins are also addressed. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and extensive references.
Comprehensive and up-to-date, Molecular Diagnosis of Infectious Diseases, Second Edition offers novice investigators an easy entry into the field and experienced researchers a broad array of time saving tools that will not only elucidate new principles, but also greatly facilitate the development and successful application of rapid diagnostic methods.

Editors and affiliations

  • Jochen Decler
    • 1
  • Udo Reischl
    • 2
  1. 1.Accenture GmbHMunichGermany
  2. 2.Institute for Medical Microbiology and HygieneUniversity of RegensburgRegenburgGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-221-6
  • Online ISBN 978-1-59259-679-9
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site