Molecular Methods in Developmental Biology

Xenopus and Zebrafish

  • Matthew Guille

Part of the Methods in Molecular Biology™ book series (MIMB, volume 127)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Jeremy Green
    Pages 1-13
  3. Toshiro Mizuno, Minori Shinya, Hiroyuki Takeda
    Pages 15-28
  4. Craig S. Newman, Paul A. Krieg
    Pages 29-40
  5. Joanne Broadbent, E. Mary Read
    Pages 57-67
  6. Rachel Macdonald
    Pages 77-88
  7. Carl Robinson, Matt Guille
    Pages 89-97
  8. Wendy Moore, Matt Guille
    Pages 99-109
  9. Qiling Xu
    Pages 125-132
  10. Ondine Cleaver, Paul A. Krieg
    Pages 133-153
  11. Jos Joore
    Pages 155-166
  12. Yuchang Fu, Donghui Kan, Sylvia Evans
    Pages 167-173
  13. Rob Orford, Darren Mernagh, Matt Guille
    Pages 187-198
  14. David Warshawsky, Leo Miller
    Pages 199-212
  15. Back Matter
    Pages 213-217

About this book

Introduction

In Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Wherever possible, these experimental approaches are applied to both Xenopus and zebrafish.
Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have fine-tuned them for best results, and a plethora of time-saving tips. State-of-the-art and readily reproducible, these powerful methods constitute today's gold-standard laboratory manual for understanding the interactions responsible for development.

Editors and affiliations

  • Matthew Guille
    • 1
  1. 1.School of Biological SciencesUniversity of PortsmouthPortsmouthUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592596789
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-790-8
  • Online ISBN 978-1-59259-678-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book