RNA-Protein Interaction Protocols

  • Susan R. Haynes

Part of the Methods in Molecular Biology™ book series (MIMB, volume 118)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Melissa J. Moore
    Pages 11-19
  3. Michelle M. Hanna, Lori Bentsen, Michael Lucido, Archana Sapre
    Pages 21-33
  4. Zhuying Wang, Tariq M. Rana
    Pages 49-62
  5. Bernd Thiede, Henning Urlaub, Helga Neubauer, Brigitte Wittmann-Liebold
    Pages 63-72
  6. Kathleen B. Hall, James K. Kranz
    Pages 105-114
  7. Christopher D. Cilley, James R. Williamson
    Pages 129-141
  8. Robert J. Crouch, Makoto Wakasa, Mitsuru Haruki
    Pages 143-160
  9. Daniel J. Kenan, Jack D. Keene
    Pages 217-231
  10. Paramjeet S. Bagga, Jeffrey Wilusz
    Pages 245-256
  11. Philippa J. Webster, Paul M. Macdonald
    Pages 257-264

About this book

Introduction

In RNA-Protein Interaction Protocols, highly skilled experimentalists provide a comprehensive series of commonly used, as well as specialized, techniques for analyzing how proteins and RNA interact. Richly detailed and readily reproducible, these methods enable researchers to analyze the structural details of an RNA-protein interaction, to determine in detail what parts of the protein and RNA are in close contact, and to isolate RNP complexes from cells. There are also in vitro and in vivo methods and protocols for assaying the effects of proteins and RNP complexes on mRNA metabolism. The methods assume only a knowledge of basic molecular biology, biochemistry, and cell culture, and can be readily adapted to other systems.
Providing an excellent standard resource for today's bench scientists studying many types of RNA-protein interactions, RNA-Protein Interaction Protocols offers a wide-ranging collection of up-to-date experimental methods that distill a great deal of wisdom and experience into productive, reproducible, step-by-step techniques for all investigators in the field.

Editors and affiliations

  • Susan R. Haynes
    • 1
  1. 1.Uniformed Services University of the Health SciencesBethesda

Bibliographic information

  • DOI https://doi.org/10.1385/1592596762
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-568-3
  • Online ISBN 978-1-59259-676-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book