Antibody Engineering

Methods and Protocols

  • Benny K. C. Lo

Part of the Methods in Molecular Biology™ book series (MIMB, volume 248)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Antibody Sequence And Structure

    1. Front Matter
      Pages 1-1
    2. Benny K. C. Lo, Yu Wai Chen
      Pages 3-9
    3. George Johnson, Tai Te Wu
      Pages 11-25
    4. Nicholas Whitelegg, Anthony R. Rees
      Pages 51-91
    5. Stephen E. Harding, Emma Longman, Beatriz Carrasco, Alvaro Ortega, Jose Garcia de la Torre
      Pages 93-113
  3. Antibody-Lead Generation

    1. Front Matter
      Pages 115-115
    2. James D. Marks, Andrew Bradbury
      Pages 117-134
    3. Benny K. C. Lo
      Pages 135-159
    4. James D. Marks, Andrew Bradbury
      Pages 161-176
    5. Mingyue He, Neil Cooley, Alison Jackson, Michael J. Taussig
      Pages 177-189
    6. C. Geoffrey Davis, Xiao-Chi Jia, Xiao Feng, Mary Haak-Frendscho
      Pages 191-200
    7. Roland E. Kontermann, Tina Völkel, Tina Korn
      Pages 227-242
  4. Antibody Expression And Optimization

    1. Front Matter
      Pages 243-243
    2. Paul J. Yazaki, Anna M. Wu
      Pages 255-268
    3. Mary C. Guttieri, Mifang Liang
      Pages 269-299
    4. Eva Stoger, Stefan Schillberg, Richard M. Twyman, Rainer Fischer, Paul Christou
      Pages 301-318
    5. Kevin Brady, Benny K. C. Lo
      Pages 319-326
    6. Syed V. S. Kashmiri, Roberto De Pascalis, Noreen R. Gonzales
      Pages 361-376
  5. Antibody Characterization and Novel Applications

    1. Front Matter
      Pages 377-377
    2. Andrea Murray, C. Rosamund L. Graves, Kevin Brady, Benny K. C. Lo
      Pages 379-388
    3. Robert Karlsson, Anita Larsson
      Pages 389-415
    4. Alexandra Huhalov, Daniel I. R. Spencer, Kerry A. Chester
      Pages 465-480
    5. Ilse Novak-Hofer, Robert Waibel, Kurt Zimmermann, Jürgen Grünberg, Kerry A. Chester, Andrea Murray et al.
      Pages 481-494
    6. Paloma de Prada, Donald W. Landry
      Pages 495-501
    7. Ira Pastan, Richard Beers, Tapan K. Bera
      Pages 503-518
    8. Andrew R. M. Bradbury, Nileena Velappan, Vittorio Verzillo, Milan Ovecka, Roberto Marzari, Daniele Sblattero et al.
      Pages 519-546
    9. Sotiris Missailidis
      Pages 547-555
  6. Back Matter
    Pages 557-561

About this book


With 10 recent FDA approvals of therapeutic antibody drugs and hundreds more in the biotechnology pipeline, it is clear that engineered antibodies will play a major role in treating human diseases in the 21st century. In Antibody Engineering: Methods and Protocols, leading researchers and clinicians present a core collection of cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the generation of human recombinant antibodies by humanization approaches, molecular display technologies, and transgenic animals. The techniques for antibody expression and optimization utilize not only bacterial and mammalian cell cultures, but also insect cell cultures and transgenic plants. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics. Each fully tested protocol is described in step-by-step detail and includes background material, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls.
Comprehensive and up-to-date, Antibody Engineering: Methods and Protocols provides both laboratory and clinical investigators with a diverse array of the fundamental techniques upon which new and imaginative drug technologies can be successfully developed.

Editors and affiliations

  • Benny K. C. Lo
    • 1
  1. 1.MRC Laboratory of Molecular BiologyUniversity of CambridgeCambridgeUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-092-2
  • Online ISBN 978-1-59259-666-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site