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Pediatric Hematology

Methods and Protocols

  • Nicholas J. Goulden
  • Colin G. Steward

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 91)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Bone Marrow Failure Syndromes

    1. Front Matter
      Pages 1-1
    2. Alex J. Tipping, Tom J. Vulliamy, Neil V. Morgan, Inderjeet Dokal
      Pages 3-17
    3. Sarah Ball, Karen Orfali
      Pages 19-30
  3. Red Blood Cell Disorders

    1. Front Matter
      Pages 31-31
  4. Platelet and Hemostatic Disorders

  5. Immunodeficiency

    1. Front Matter
      Pages 89-89
    2. David Eastwood, Kimberly C. Gilmour, Hubert B. Gaspar
      Pages 91-108
  6. Acute Leukemia

    1. Front Matter
      Pages 109-109
    2. Frederik W. van Delft, Vaskar Saha
      Pages 111-122
    3. Christine J. Harrison, Helena Kempski, David W. Hammond, Lyndal Kearney
      Pages 123-137
    4. Cornelia Eckert, Olfert Landt
      Pages 175-182
    5. Frederik W. van Delft, Louise K. Jones
      Pages 183-196
  7. Bone Marrow Transplantation

    1. Front Matter
      Pages 197-197
    2. John Harvey, Jenny Price, Ahna Stanton
      Pages 199-232
    3. Paul P. J. Dunn, Sarinder Day, Siaron Williams, Nina Bendukidze
      Pages 233-246
    4. Peter Bader, Hermann Kreyenberg
      Pages 247-264
  8. Back Matter
    Pages 265-272

About this book

Introduction

Molecular techniques are now an integral part of the practice of modern pediatric hematology. In Pediatric Hematology: Methods and Protocols, hands-on masters of laboratory techniques describe their best methods for investigating and detecting a wide variety of hematological disorders. Here, the reader will find cutting-edge molecular protocols for the diagnosis of Fanconi anemia, dyskeratosis congenita, immunodeficiency, and most forms of hemoglobinopathy. In addition, there are detailed methods for molecular human platelet antigen genotyping, an effective PCR procedure for thrombophilia screening, and protocols for fluorescent in situ hybridization for cases in which insufficient metaphases are recovered or cryptic translocations are not visible. Since the measurement of minimal residual disease (MRD) provides a much more accurate risk-directed therapy, three methods are presented for detecting residual leukemia below the threshold of light microscopy, along with relatively simple, rapid, and inexpensive methods for the detection of MRD in ALL and AML. On the cutting-edge of technology, microarrays are used for the analysis of gene expression in childhood leukemia, and novel methods of graft engineering and reduced intensity conditioning regimens are applied to tissue typing for the assessment of donor/recipient chimerism. Each readily reproducible protocol is described in step-by-step detail and includes an introduction to the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Pediatric Hematology: Methods and Protocols offers experimental and clinical investigators a gold-standard collection of reliable molecular techniques essential to the diagnosis, classification, and treatment of childhood hematological disorders.

Editors and affiliations

  • Nicholas J. Goulden
    • 1
  • Colin G. Steward
    • 1
  1. 1.Department of Paediatric Haematology, Oncology, and Bone Marrow TransplantationRoyal Hospital for ChildrenBristolUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592594336
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-043-4
  • Online ISBN 978-1-59259-433-7
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site