G Protein Signaling

Methods and Protocols

  • Alan V. Smrcka

Part of the Methods in Molecular Biology™ book series (MIMB, volume 237)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Purification of G Protein Subunits and G Protein Effectors

  3. Assays for G Protein—Dependent Effector Activation In Vitro

  4. Assays For G Protein-Coupled Receptor-Mediated and G Protein Subunit-Mediated Effector Activation in Intact Cells

  5. Receptor Mechanisms, Activity, and Localization

  6. Disruption of Endogenous G Protein-Signaling Systems to Understand the Role of G Protein Subunits in Intact Systems

    1. Front Matter
      Pages 151-151
    2. Janet D. Robishaw, Zheng-ping Guo, Qin Wang
      Pages 169-180
  7. Posttranslational Modifications of Regulators of G Protein Signaling

    1. Front Matter
      Pages 193-193
    2. Leah S. Bernstein, Maurine E. Linder, John R. Hepler
      Pages 195-204
  8. Use of Gfp-Tagged G Proteins and Effectors in Intact Cells

    1. Front Matter
      Pages 221-221
    2. Thomas R. Hynes, Thomas E. Hughes, Catherine H. Berlot
      Pages 233-246
  9. Back Matter
    Pages 247-249

About this book


G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. The authors focus on the mechanisms of G protein and G protein-coupled receptor function and the roles of G protein subunits in cell biology and disease. Among the readily reproducible techniques presented are those for the purification of G proteins and effector enzymes, assays of these purified G proteins and effector enzymes, and for the study of G protein interactions with effectors in intact cells. Additional methods are provided for assaying G protein-coupled receptor structure, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Comprehensive and highly practical, G Protein Signaling: Methods and Protocols offers novice and expert researchers alike an array of cutting-edge tools to illuminate the roles of G protein and G protein-coupled receptors, as well as a better understanding of their action with drugs, neurotransmitters, and sensory stimuli.

Editors and affiliations

  • Alan V. Smrcka
    • 1
  1. 1.Department of Pharmacology and PhysiologyUniversity of Rochester School of MedicineRochester

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-137-0
  • Online ISBN 978-1-59259-430-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site