Plant Functional Genomics

  • Erich Grotewold

Part of the Methods in Molecular Biology™ book series (MIMB, volume 236)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Finding Genes in Complex Plant Systems

    1. Front Matter
      Pages 1-1
    2. Meizhong Luo, Rod A. Wing
      Pages 3-19
    3. Manish N. Raizada
      Pages 37-58
    4. Isabelle M. Henry, Dina F. Mandoli
      Pages 59-78
    5. Edgar Huitema, Trudy A. Torto, Allison Styer, Sophien Kamoun
      Pages 79-83
  3. In Silico Prediction of Plant Gene Function

    1. Front Matter
      Pages 85-85
    2. Ramana V. Davuluri, Michael Q. Zhang
      Pages 87-107
    3. Wusirika Ramakrishna, Jeffrey L. Bennetzen
      Pages 109-121
    4. Sherry R. Whitt, Edward S. Buckler IV
      Pages 123-139
  4. Forward and Reverse Genetic Strategies

    1. Front Matter
      Pages 155-155
    2. Thomas P. Brutnell, Liza J. Conrad
      Pages 157-175
    3. Jose M. Alonso, Anna N. Stepanova
      Pages 177-187
    4. Andrea Kodym, Rownak Afza
      Pages 189-203
    5. Bradley J. Till, Trenton Colbert, Rachel Tompa, Linda C. Enns, Christine A. Codomo, Jessica E. Johnson et al.
      Pages 205-220
    6. Marcela Rojas-Pierce, Patricia S. Springer
      Pages 221-240
    7. Susan Varsha Wesley, Qing Liu, Anna Wielopolska, Geoff Ellacott, Neil Smith, Surinder Singh et al.
      Pages 273-286
    8. S. P. Dinesh-Kumar, Radhamani Anandalakshmi, Rajendra Marathe, Michael Schiff, Yule Liu
      Pages 287-293
    9. Dileep K. Pulukkunat, M. L. Stephen Raj, Debasis Pattanayak, Lien B. Lai, Venkat Gopalan
      Pages 295-309
    10. Randy Scholl, Martin M. Sachs, Doreen Ware
      Pages 311-326
  5. Gain of Function Approaches

    1. Front Matter
      Pages 327-327
    2. Johan Memelink
      Pages 345-361
  6. Phenotypic Profiling as a Tool to Determine Gene Function

    1. Front Matter
      Pages 363-363
    2. Suling Zhao, Wesley B. Bruce
      Pages 365-380
    3. Ulrike Mathesius, Nijat Imin, Siria H. A. Natera, Barry G. Rolfe
      Pages 395-413
    4. Anusha P. Dias, Johnny Brown, Pierluigi Bonello, Erich Grotewold
      Pages 415-425
    5. Susanne Kjemtrup, Douglas C. Boyes, Cory Christensen, Amy J. McCaskill, Michelle Hylton, Keith Davis
      Pages 427-441
  7. Back Matter
    Pages 443-449

About this book


As more and more plant genomes are sequenced, plant researchers have been inundated with an avalanche of novel methodologies to identify the function of tens of thousands of plant genes. In Plant Functional Genomics, Erich Grotewold has assembled a team of leading plant scientists to describe in detail the most commonly used methods for investigating plant gene function in a wide variety of plants, during plant pathogen interactions, and even in algae. These readily reproducible protocols include computational, molecular, and genetic methodologies designed for both general and specific problems. Here the reader will learn how to identify genes in complex systems that have large genomes, few cells, and mixed cell systems. Readers will also learn the use of powerful computational and statistical tools to help predict gene function, either on the basis of comparative genomics, or from the analysis of complex genome sequences. Because establishing gene function relies on the identification of phenotypes, the authors expand the concept of phenotypes, including the use of multiple outputs as the ultimate phenotypic result of changes in gene activity. Numerous loss-of-function and gain-of-function techniques for discovering gene function are presented in step-by-step detail.
Comprehensive and highly practical, Plant Functional Genomics offers plant biologists readily reproducible computational, molecular, and genetic protocols, powerful tools that will enable them, with little or no experience, successfully to investigate any gene function with the most recent methodologies.

Editors and affiliations

  • Erich Grotewold
    • 1
  1. 1.Department of Plant Biology and Plant Biotechnology CenterThe Ohio State UniversityColumbus

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-145-5
  • Online ISBN 978-1-59259-413-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site