Book Volume 232 2003

Protein Misfolding and Disease

Principles and Protocols

Editors:

ISBN: 978-1-58829-065-6 (Print) 978-1-59259-394-1 (Online)

Table of contents (24 protocols)

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  1. Front Matter

    Pages i-xv

  2. General Concepts and Models

    1. Front Matter

      Pages 1-1

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      Protocol

      Pages 3-16

      Protein Misfolding, Aggregation, and Degradation in Disease

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      Protocol

      Pages 17-26

      Basic Introduction to In Vivo Protein Folding and Its Defects

    4. No Access

      Protocol

      Pages 27-37

      Cystic Fibrosis

    5. No Access

      Protocol

      Pages 39-56

      α1-Antitrypsin Deficiency

    6. No Access

      Protocol

      Pages 57-66

      Parkinson’s Disease

    7. No Access

      Protocol

      Pages 67-76

      Aberrant Protein Folding as the Molecular Basis of Cancer

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      Protocol

      Pages 77-90

      Yeast as a Model System to Investigate Protein Conformational Diseases

  3. General Methods

    1. Front Matter

      Pages 91-91

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      Protocol

      Pages 93-101

      Expression of Recombinant Proteins

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      Protocol

      Pages 103-109

      Expression in E. coli Systems

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      Protocol

      Pages 111-125

      Protein Expression in Yeasts

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      Protocol

      Pages 127-131

      Site-Directed Mutagenesis

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      Protocol

      Pages 133-145

      Pulse-Chase Labeling Techniques for the Analysis of Protein Maturation and Degradation

  4. Techniques in Conformational Disease Research

    1. Front Matter

      Pages 147-147

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      Protocol

      Pages 149-164

      Detection of Aggregates and Protein Inclusions by Staining of Tissues

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      Protocol

      Pages 165-173

      Study of Mutant Proteins With Folding Defects in Cultured Patient Cells

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      Protocol

      Pages 175-182

      E. coli Expression System for Identifying Folding Mutations of Human Adenosine Deaminase

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      Protocol

      Pages 183-202

      Characterization of Overexpressed Mutant Proteins in Mammalian Cells

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      Protocol

      Pages 203-216

      Investigation of Unfolded-Protein Response in Cells Expressing Familial Alzheimer's Disease-Linked Presenilin Variants

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      Protocol

      Pages 217-229

      Huntingtin Fragments Form Aggresome-Like Inclusion Bodies in Mammalian Cells

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      Protocol

      Pages 231-243

      Application of Chemical Chaperones to the Rescue of Folding Defects

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      Protocol

      Pages 245-256

      Analysis of Defective Subunit Interactions Using the Two-Hybrid System

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