Lentivirus Gene Engineering Protocols

  • Maurizio Federico

Part of the Methods in Molecular Biology™ book series (MIMB, volume 229)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Lentivirus Vectors

    1. Front Matter
      Pages 1-1
    2. Maurizio Federico
      Pages 3-15
    3. Francesco Lotti, Fulvio Mavilio
      Pages 17-27
    4. Annmarie L. Pacchia, Sayandip Mukherjee, Joseph P. Dougherty
      Pages 29-42
    5. Michael P. Marino, Milson J. Luce, Jakob Reiser
      Pages 43-55
    6. Joel Gatlin, Miguel Islas-Ohlmayer, J. Victor Garcia
      Pages 57-68
    7. Harry Segall, Richard E. Sutton
      Pages 87-94
  3. Transduction of EX Vivo Cell Cultures Through Lentivirus Vectors

    1. Front Matter
      Pages 95-95
    2. Dhanalakashmi Chinnasamy, Fabio Candotti
      Pages 97-105
    3. Harry Segall, Richard E. Sutton
      Pages 107-116
    4. Robert E. Donahue, Irvin S. Y. Chen
      Pages 117-130
    5. Xian-Yang Zhang, Vincent F. La Russa, Jakob Reiser
      Pages 131-140
    6. Jay L. Nadeau
      Pages 141-154
    7. Jane Fleming, Samantha L. Ginn, Ian E. Alexander
      Pages 155-167
    8. Dèsirèe Bonci, Michael V. G. Latronico, Gianluigi Condorelli
      Pages 169-179
    9. Larry G. Johnson, Scott H. Randell, John C. Olsen
      Pages 181-196
    10. Binoy Appukuttan, Trevor McFarland, J. Timothy Stout
      Pages 197-203
    11. Trevor McFarland, Binoy Appukuttan, J. Timothy Stout
      Pages 205-216
  4. Advantages and Uses of Non-HIV-1-Based Lentivirus Vectors

    1. Front Matter
      Pages 217-217
    2. Suresh K. Arya, Jean E. Cho, Suzie H. Chang
      Pages 219-231
    3. Alessia Ruggieri, Didier Nègre, François-Loïc Cosset
      Pages 233-249
    4. Nils Loewen, Roman Barraza, Todd Whitwam, Dyana T. Saenz, Iris Kemler, Eric M. Poeschla
      Pages 251-271
    5. Vicente Planelles
      Pages 273-284
  5. Direct In Vivo Transduction by Lentivirus Vector and Detection

    1. Front Matter
      Pages 285-285
    2. Patrick L. Sinn, Paul B. McCray Jr.
      Pages 287-298
    3. Carl Rosenblad, Cecilia Lundberg
      Pages 299-307
  6. Back Matter
    Pages 309-314

About this book


Lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with detailed methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. The use of non-HIV-1-based lentivirus vectors is shown to be advantageous, particularly those obtained from the genomes of HIV-2, SIV, or FIV. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit.
Comprehensive and informative, Lentivirus Gene Engineering Protocols presents a broad spectrum of the latest methods of lentivirus vector technology, offering hematologists, neurologists, geneticists, and virologists the most promising and up-to-date tools available for gene therapy in such still fatal diseases as cancer, cystic fibrosis, and AIDS.

Editors and affiliations

  • Maurizio Federico
    • 1
  1. 1.Laboratory of VirologyIstituto Superiore di SanitáRomeItaly

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-091-5
  • Online ISBN 978-1-59259-393-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site