Superantigen Protocols

  • Teresa Krakauer

Part of the Methods in Molecular Biology™ book series (MIMB, volume 214)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Matthew D. Baker, K. Ravi Acharya
    Pages 1-31
  3. John K. McCormick, Patrick M. Schlievert
    Pages 33-43
  4. Anders Cavallin, Karin Petersson, Göran Forsberg
    Pages 55-63
  5. S.Munir Alam, Nicholas R. J. Gascoigne
    Pages 65-85
  6. Hywyn R. O. Churchill, David M. Kranz
    Pages 87-99
  7. Hywyn R. O. Churchill, David M. Kranz
    Pages 101-111
  8. Catherine Gelin, Marie-Thérèse Zilber, Dominique Charron
    Pages 113-126
  9. Anne Roumier, Florence Niedergang, Andrés Alcover
    Pages 127-136
  10. Natalie Sutkowski, Brigitte T. Huber
    Pages 185-218
  11. John K. McCormick, Gregory A. Bohach, Patrick M. Schlievert
    Pages 245-254
  12. Back Matter
    Pages 255-259

About this book


Superantigens, a class of proteins produced by bacteria and microbes, cause diseases ranging from food poisoning to toxic shock, but can also be used to understand these pathologies and devise therapeutic agents to combat them. In Superantigen Protocols, leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of these potent activators of the immune system. The protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level. Additional techniques for the discovery of new activators of the immune system, the development of new therapeutics for the suppression of superantigen-induced effects, and the treatment of inflammatory diseases are detailed.
Comprehensive and cutting-edge, Superantigen Protocols provides a broad range of novel immunological and biochemical techniques that can be successfully applied not only to the structural, functional, and clinical properties of superantigens, but also to the many varieties of such proteins and to new cellular systems.

Editors and affiliations

  • Teresa Krakauer
    • 1
    • 2
  1. 1.Uniformed Services University of the Health SciencesBethesda
  2. 2.US Army Medical Research Institute of Infectious DiseasesFrederick

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-984-1
  • Online ISBN 978-1-59259-367-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site