Bone Research Protocols

  • Miep H. Helfrich
  • Stuart H. Ralston

Part of the Methods in Molecular Medicine book series (MIMM, volume 80)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Culture of Osteolblasts and Osteocytes

    1. Front Matter
      Pages 1-1
    2. James A. Gallagher
      Pages 3-18
    3. Astrid Bakker, Jenneke Klein-Nulend
      Pages 19-28
    4. Andrew Scutt, Lynsey Reading, Nanette Scutt, Karen Still
      Pages 29-39
    5. Peter J. Nijweide, Arie van der Plas, Marcel J. Alblas, Jenneke Klein-Nulend
      Pages 41-50
  3. Culture of Osteoclasts

    1. Front Matter
      Pages 51-51
    2. Astrid Hoebertz, Timothy R. Arnett
      Pages 53-64
    3. Patricia Collin-Osdoby, Fred Anderson, Philip Osdoby
      Pages 65-88
    4. Fraser P. Coxon, Julie C. Frith, Helena L. Benford, Michael J. Rogers
      Pages 89-99
    5. Afsie Sabokbar, Nicholas S. Athanasou
      Pages 101-111
    6. Adrienne M. Flanagan, Helen M. Massey
      Pages 113-128
    7. Naoyuki Takahashi, Nobuyuki Udagawa, Sakae Tanaka, Tatsuo Suda
      Pages 129-144
    8. Robert J. van’t Hof
      Pages 145-152
    9. Patricia Collin-Osdoby, Xuefeng Yu, Hong Zheng, Philip Osdoby
      Pages 153-166
  4. Bone Organ Culture

    1. Front Matter
      Pages 167-167
    2. Michael J. Marshall, Marit N. Rowlands
      Pages 169-182
  5. Histological and Ultrastructural Techniques

    1. Front Matter
      Pages 199-199
    2. Brendon S. Noble, Hazel Y. Stevens
      Pages 225-236
    3. Stephen A. Nesbitt, Michael A. Horton
      Pages 259-281
    4. Shobna Vedi, Juliet Compston
      Pages 283-298
    5. Vincent Everts, Anneke Niehof, Wouter Beertsen
      Pages 299-310
    6. Deborah Marshall, Miep H. Helfrich, Richard M. Aspden
      Pages 311-320
  6. Imaging Techniques

    1. Front Matter
      Pages 321-321
  7. In Vivo Techniques

    1. Front Matter
      Pages 343-343
    2. Robert J. van’t Hof, Claire E. Clarkin, Kenneth J. Armour
      Pages 345-351
    3. Kenneth J. Armour, Katharine E. Armour
      Pages 353-360
    4. Jade W. M. Chow
      Pages 361-365
  8. Mechanical Loading Techniques

    1. Front Matter
      Pages 367-367
    2. Richard M. Aspden
      Pages 369-379
    3. Hazel Y. Stevens, John A. Frangos
      Pages 381-398
    4. Andrew A. Pitsillides, Victoria Das-Gupta, Dominic Simon, Simon C. F. Rawlinson
      Pages 399-422
  9. Molecular Analysis

    1. Front Matter
      Pages 423-423
    2. Tracy L. Stewart, Val Mann
      Pages 425-432
  10. Back Matter
    Pages 441-448

About this book


The past decade has witnessed the discovery of many genes with profound effects on bone. In Bone Research Protocols, recognized authorities with extensive hands-on experience present a wide-ranging catalog of laboratory techniques that will help researchers to investigate the mechanisms by which these genes affect bone mass and strength. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone. Though in vitro methods receive central attention, there are also protocols for in vivo analyses of bone formation and resorption.
State-of-the-art and highly practical, Bone Research Protocols offers both beginning and advanced laboratory researchers a major compendium of well-established techniques for studying bone cell function and metabolism.

Editors and affiliations

  • Miep H. Helfrich
    • 1
  • Stuart H. Ralston
    • 1
  1. 1.University of Aberdeen Medical SchoolAberdeenUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-044-1
  • Online ISBN 978-1-59259-366-8
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site