PCR Detection of Microbial Pathogens

  • Konrad Sachse
  • Joachim Frey

Part of the Methods in Molecular Biology™ book series (MIMB, volume 216)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Reviews

    1. Front Matter
      Pages 1-1
    2. Peter Rådström, Rickard Knutsson, Petra Wolffs, Maria Dahlenborg, Charlotta Löfström
      Pages 31-50
    3. Jeffrey Hoorfar, Nigel Cook
      Pages 51-64
    4. Peter Stephensen Lübeck, Jeffrey Hoorfar
      Pages 65-84
  3. Protocols

    1. Front Matter
      Pages 85-1
    2. Mark D. Englen, Scott R. Ladely, Paula J. Fedorka-Cray
      Pages 109-121
    3. Konrad Sachse, Helmut Hotzel
      Pages 123-136
    4. Michel R. Popoff
      Pages 137-152
    5. Mustapha Berri, Nathalie Arricau-Bouvery, Annie Rodolakis
      Pages 153-161
    6. John W. Lester, Rance B. LeFebvre
      Pages 193-200
    7. Robin A. Skuce, M. Siobhan Hughes, Malcolm J. Taylor, Sydney D. Neill
      Pages 201-221
    8. Mazhar I. Khan
      Pages 223-230
    9. Helmut Hotzel, Joachim Frey, John Bashiruddin, Konrad Sachse
      Pages 231-245
    10. Marylène Kobisch, Joachim Frey
      Pages 247-256

About this book

Introduction

Because the number of published PCR procedures is already large and rising steadily, there is a need to critically evaluate and eventually standardize its methodology so that laboratory diagnosticians can select optimal techniques for their own work. In PCR Detection of Microbial Pathogens, hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. Described in step-by-step detail to ensure successful experimental results, these proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis. Each article details a validated technique that explains the principles involved, provides stepwise instructions and a list of materials, and includes notes describing problems that may arise, as well as possible alternative procedures.
Authoritative and easy-to-use, PCR Detection of Microbial Pathogens offers microbiologists, veterinary diagnosticians, and food analysts powerful state-of-the-art tools designed not only to expand their knowledge of the potential of DNA-based diagnostic methods, but also to help select those techniques best suited to their specific practical needs.

Editors and affiliations

  • Konrad Sachse
    • 1
  • Joachim Frey
    • 2
  1. 1.Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV)JenaGermany
  2. 2.University of BernBernSwitzerland

Bibliographic information

  • DOI https://doi.org/10.1385/1592593445
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-049-6
  • Online ISBN 978-1-59259-344-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book