Protein Sequencing Protocols

  • Bryan John Smith

Part of the Methods in Molecular Biology™ book series (MIMB, volume 211)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Bryan John Smith, Paul Tempst
    Pages 1-18
  3. Bryan John Smith
    Pages 49-61
  4. Bryan John Smith
    Pages 63-82
  5. G. Brent Irvine, Ian Davidson
    Pages 123-131
  6. Alan J. Smith
    Pages 133-141
  7. Andrea Scaloni, Maurizio Simmaco, Francesco Bossa
    Pages 169-180
  8. Andrew J. Reason
    Pages 181-194
  9. Fiona M. Greer, Howard R. Morris
    Pages 195-210
  10. David M. Schieltz, John R. Yates III
    Pages 235-245
  11. Gregory A. Grant, Mark W. Crankshaw
    Pages 247-268
  12. Laurey Steinke, Richard G. Cook
    Pages 301-307
  13. Philip J. Jackson, Neil Dodsworth
    Pages 309-318
  14. David R. Dupont, Sylvia W. Yuen, Kenneth S. Graham
    Pages 319-331
  15. Hisashi Hirano, Roza M. Kamp
    Pages 355-363
  16. David J. Harvey
    Pages 371-383
  17. Alastair Aitken, Michele Learmonth
    Pages 385-398
  18. Alastair Aitken, Michele Learmonth
    Pages 399-410
  19. Richard R. Copley, Robert B. Russell
    Pages 411-430
  20. Alastair Aitken
    Pages 465-485
  21. Back Matter
    Pages 487-493

About this book


Bryan John Smith updates his much-acclaimed first edition of Protein Sequencing Protocols with new and refreshed techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to the analysis of protein sequence information via the powerful new tools of bioinformatics. Each readily reproducible protocol contains detailed step-by-step instructions to ensure successful experimental results, along with notes on troubleshooting and how to avoid pitfalls. Valuable appendices summarize changes in mass arising from protein modification, as well as consensus sequences for protein modification.
Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination and thus help assure robust results.

Editors and affiliations

  • Bryan John Smith
    • 1
  1. 1.Celltech ChiroscienceSloughUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-975-9
  • Online ISBN 978-1-59259-342-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site