Recombinant Antibodies for Cancer Therapy

Methods and Protocols

  • Martin Welschof
  • Jürgen Krauss

Part of the Methods in Molecular Biology™ book series (MIMB, volume 207)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Jürgen Krauss, Michaela Arndt, Michael Pfreundschuh
      Pages 27-53
  3. Hybridoma-Derived Antibodies

    1. Front Matter
      Pages 55-55
    2. Jürgen Krauss, Hans Heinrich Förster, Barbara Uchanska-Ziegler, Andreas Ziegler
      Pages 63-79
    3. Siobhan O’Brien, Tarran Jones
      Pages 81-100
  4. Recombinant Antibody Fragments from Phagemid-Displayed Antibody Repertoires

    1. Front Matter
      Pages 101-101
    2. Martin Welschof, Christiane Christ, Ingrid Hermes, Armin Keller, Christian Kleist, Michael Braunagel
      Pages 103-121
    3. Michael Braunagel
      Pages 123-132
    4. Liat Binyamin, Daniel Plaksin, Yoram Reiter
      Pages 133-143
    5. Mariangela Figini, Andrew Green, Francesco Colotta, Silvana Canevari
      Pages 145-159
    6. Jan Engberg, Ali F. Yenidunya, Rikke Clausen, Liselotte B. Jensen, Peter Sørensen, Pernille Kops et al.
      Pages 161-177
  5. Antibody Fragments with Additional Properties

    1. Front Matter
      Pages 235-235
    2. Partha S. Chowdhury, George Vasmatzis
      Pages 237-254

About this book

Introduction

Many antibodies recently developed as therapeutic agents have been tailored by the powerful new recombinant antibody technology, and already their clinical data show highly encouraging results. In Recombinant Antibodies for Cancer Therapy: Methods and Protocols, Martin Welschof and Jürgen Krauss present a collection of carefully selected protocols for the design, construction, and characterization of those novel anticancer therapeutics. Written by seasoned investigators who have successfully verified the particular method described, these readily reproducible methods include coverage of hybridoma-derived recombinant antibodies, recombinant antibody fragments from phagemid-displayed antibody repertoires, antibody fragments with additional properties, and large-scale production of recombinant antibodies for clinical applications. The main focus is on providing detailed protocols that describe these procedures step-by-step, accompanied by a troubleshooting guide discussing possible problems and offering many tips for their potential solutions. Concise review articles also survey the current status of recombinant antibodies in cancer therapy, as well as the generation of antibody molecules through antibody engineering.
Up-to-date and highly practical, Recombinant Antibodies for Cancer Therapy: Methods and Protocols surveys the current status of engineered antibodies and describes in rich detail many readily reproducible methods of generating the novel and promising antibody-based reagents of today's cancer therapy.

Editors and affiliations

  • Martin Welschof
    • 1
  • Jürgen Krauss
    • 2
  1. 1.Axaron Bioscience AGHeidelbergGermany
  2. 2.National Institutes of HealthNational Cancer InstituteFrederick

Bibliographic information

  • DOI https://doi.org/10.1385/1592593348
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-918-6
  • Online ISBN 978-1-59259-334-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book