Table of contents

  1. Front Matter
    Pages i-xi
  2. Gail G. Hardy, Simone M. Tudor, Joseph W. St. Geme III
    Pages 1-28
  3. Grant Poje, Rosemary J. Redfield
    Pages 51-56
  4. Grant Poje, Rosemary J. Redfield
    Pages 57-70
  5. Alex van Belkum, Loek van Alphen
    Pages 71-92
  6. Ioanna D. Dimopoulou, Mohd-Zain Zaini, Derrick W. Crook
    Pages 93-99
  7. Phillip Cash, J. Simon Kroll
    Pages 101-118
  8. Tahir R. Ali, Ming-Shi Li, Paul R. Langford
    Pages 119-134
  9. Xavier de Bolle, Christopher D. Bayliss
    Pages 135-146
  10. Derek W. Hood
    Pages 147-160
  11. Mark A. Herbert
    Pages 185-209
  12. Stefan Schlör, Gabriele Kemmer, Joachim Reidl
    Pages 211-224
  13. Martin Handfield, Trevor Seifert, Jeffrey D. Hillman
    Pages 225-242
  14. Joyce S. Plested, Philip A. Coull, Margaret Anne J. Gidney
    Pages 243-261
  15. Joyce S. Plested, Philip A. Coull
    Pages 263-275
  16. Andrew M. Middleton, Paula Keig, Robert Wilson
    Pages 277-295
  17. Mumtaz Virji, Darryl J. Hill
    Pages 315-322
  18. Back Matter
    Pages 323-333

About this book


As the second leading bacterial cause of community-acquired pneumonia, H. influenzae accounts for many thousands of deaths per year worldwide, and is a prime candidate for the identification of targets that can lead to the development of novel vaccines and antibiotics. In Haemophilus influenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of this significant bacterium. Described with step-by-step instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. The antibody techniques presented include IVIAT, ELISA, and opsonophagocytosis assays. Also included are reviews of the pathogenesis of NTHi and Hib, methods for working with the organism, culture and storage conditions, molecular diagnosis, and systems for exploring pathogenesis in both in vitro and animal models.
Comprehensive and highly practical, Haemophilus influenzae Protocols offers a unique collection of powerful molecular methods that illuminate not only how the bacterium causes disease, but also how best to develop much-needed new vaccines and antibiotics against it.


antibiotics bacteria infection infections infectious infectious disease tissue

Editors and affiliations

  • Mark A. Herbert
    • 1
  • Derek W. Hood
    • 1
  • E. Richard Moxon
    • 1
  1. 1.Department of PediatricsJohn Radcliffe HospitalOxfordUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-928-5
  • Online ISBN 978-1-59259-321-7
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site