Viral Vectors for Gene Therapy

Methods and Protocols

  • Curtis A. Machida

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 76)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Edward A. Burton, Shaohua Huang, William F. Goins, Joseph C. Glorioso
    Pages 1-31
  3. Yoshinaga Saeki, Xandra O. Breakefield, E. Antonio Chiocca
    Pages 51-60
  4. Charles J. Link, Nicholas N. Vahanian, Suming Wang
    Pages 61-87
  5. Catherine R. O’Riordan, Antonius Song, Julia Lanciotti
    Pages 89-112
  6. Andres Hurtado-Lorenzo, Anne David, Clare Thomas, Maria G. Castro, Pedro R. Lowenstein
    Pages 113-133
  7. Hartmut Stecher, Cheryl A. Carlson, Dmitry M. Shayakhmetov, André Lieber
    Pages 135-152
  8. Jeffrey S. Chamberlain, Catherine Barjot, Jeannine Scott
    Pages 153-166
  9. Semyon Rubinchik, Jan Woraratanadharm, Jennifer Schepp, Jian-yun Dong
    Pages 167-199
  10. Matthew D. Weitzman, Samuel M. Young Jr., Toni Cathomen, Richard Jude Samulski
    Pages 201-219
  11. Matthew J. During, Deborah Young, Kristin Baer, Patricia Lawlor, Matthias Klugmann
    Pages 221-236
  12. Julian D. Harris, Stuart G. Beattie, J. George Dickson
    Pages 255-267
  13. Beverly L. Davidson, John A. Chiorini
    Pages 269-285
  14. Dongsheng Duan, Yongping Yue, Ziying Yan, John F. Engelhardt
    Pages 287-307
  15. Estanislao Bachrach, Mogens Duch, Mireia Pelegrin, Hanna Dreja, Finn Skou Pederson, Marc Piechaczyk
    Pages 343-352
  16. Biao Li, Curtis A. Machida
    Pages 353-365
  17. Deborah J. Watson, John H. Wolfe
    Pages 383-403
  18. Sybille L. Sauter, Mehdi Gasmi, Thomas W. Dubensky Jr.
    Pages 405-432
  19. Yonghong Zhu, Vicente Planelles
    Pages 433-448
  20. John C. Kappes, Xiaoyun Wu, John K. Wakefield
    Pages 449-465
  21. Jarmo Wahlfors, Richard A. Morgan
    Pages 493-502
  22. Kenneth Lundstrom, Markus U. Ehrengruber
    Pages 521-523
  23. George Vassilopoulos, Neil C. Josephson, Grant Trobridge
    Pages 545-564
  24. Back Matter
    Pages 579-589

About this book


The promise of gene therapy can be realized only if workable vectors can be found to deliver therapeutic genes. In Viral Vectors for Gene Therapy: Methods and Protocols, leading researchers from academia and biotechnology describe proven molecular methods for the construction, development, and use of virus vectors for gene transfer and gene therapy. Offering detailed step-by-step instructions to ensure successful results, these experts detail the use of herpes viruses, adenoviruses, adeno-associated viruses, simple and complex retroviruses, including lentiviruses, and other virus systems for vector development and gene transfer. Additional chapters demonstrate the use of virus vectors in the brain and central nervous system. Each protocol includes a discussion of the principles involved, numerous charts and tables, ample references, and notes on possible problems, troubleshooting, and alternative procedures.
Comprehensive and highly practical, Viral Vectors for Gene Therapy: Methods and Protocols provides not only researchers with the basic tools needed to design targeted gene delivery vectors, but also clinicians with an understanding of how to apply viral vectors to the treatment of genetic disorders.

Editors and affiliations

  • Curtis A. Machida
    • 1
  1. 1.Department of Oral Molecular BiologySchool of Dentistry Oregon Health & Science UniversityPortland

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-019-9
  • Online ISBN 978-1-59259-304-0
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site