Green Fluorescent Protein

Applications and Protocols

  • Barry W. Hicks

Part of the Methods in Molecular Biology book series (MIMB, volume 183)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Manipulation of Green Fluorescent Protein Structure at the Genetic Level

  3. Detection and Imaging of Green Fluorescent Protein

    1. Front Matter
      Pages 87-87
    2. Ammasi Periasamy, Masilamani Elangovan, Elizabeth Elliott, David L. Brautigan
      Pages 89-100
  4. Green Fluorescent Protein to Monitor Protein Distribution and Trafficking

    1. Front Matter
      Pages 149-149
    2. Henry Weiner
      Pages 171-180
  5. Green Fluorescent Protein in Transgenic Organisms

    1. Front Matter
      Pages 199-199
    2. Anthony W. S. Chan, Kowit-Yu Chong, Gerald Schatten
      Pages 201-214
    3. Ebrahim Shafizadeh, Haigen Huang, Shuo Lin
      Pages 225-234
    4. C. Neal Stewart Jr., Matthew D. Halfhill, Reginald J. Millwood
      Pages 245-252
  6. Green Fluorescent Protein Biosensors

    1. Front Matter
      Pages 253-253
    2. Anikó Váradi, Guy A. Rutter
      Pages 255-264
    3. Arjen Schots, Jan M. van der Wolf
      Pages 265-273
    4. Christopher Coker, Hui Zhao, Harry L. T. Mobley
      Pages 287-294
  7. Viral Applications of Green Fluorescent Protein

  8. Back Matter
    Pages 373-393

About this book


Green fluorescent protein (GFP), a critically important marker protein, allows direct visualization in live samples without the aid of additional sample preparation. In Green Fluorescent Protein: Applications and Protocols, Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of this marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging), for using GFP to create and select for transgenic organisms, for its use as a biosensor, and for studying viruses. Each method is described in step-by-step detail to ensure robust results and can be used in applications ranging from agriculture and botany to human gene therapy. Extensive notes review the materials required, explain the scientific basis of each step and possible sources of error, and provide tips for overcoming problems.
Highly practical and state-of-the-art, Green Fluorescent Protein: Applications and Protocols makes available to all biological and biomedical experimentalists a full array of the powerful new GFP techniques.

Editors and affiliations

  • Barry W. Hicks
    • 1
  1. 1.Department of ChemistryUnited States Air Force AcademyColorado SpringsUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-905-6
  • Online ISBN 978-1-59259-280-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site