PCR Mutation Detection Protocols

  • Bimal D. M. Theophilus
  • Ralph Rapley

Part of the Methods in Molecular Biology book series (MIMB, volume 187)

Table of contents

  1. Front Matter
    Pages i-x
  2. Andrea M. Guilliatt
    Pages 1-12
  3. Ralph Rapley, Bimal D. M. Theophilus
    Pages 13-15
  4. Adrian J. Harwood
    Pages 17-22
  5. Ralph Rapley, Ian J. Williams
    Pages 23-27
  6. Mohammad S. Enayat
    Pages 29-35
  7. PCR
    Nicola Louise Jones
    Pages 37-46
  8. Elaine K. Green
    Pages 47-50
  9. Peter A. Davies, George Gray
    Pages 51-55
  10. Anu Wartiovaara, Ann-Christine Syvänen
    Pages 57-63
  11. G. K. Surdhar
    Pages 65-72
  12. Sara A. Dyer, Elaine K. Green
    Pages 73-86
  13. Carol A. Hardy
    Pages 87-108
  14. Yvonne Wallis
    Pages 125-135
  15. Ian J. Williams, Anne C. Goodeve
    Pages 137-150
  16. Andrew J. Wallace
    Pages 151-163
  17. Christian Jurinke, Dirk van den Boom, Charles R. Cantor, Hubert Köster
    Pages 179-192
  18. Henry Brzeski
    Pages 193-208
  19. Back Matter
    Pages 209-212

About this book


Application of DNA technology to the identification of disease-causing mutations has become widespread in recent years. PCR Mutation Detection Protocols, provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Additional coverage is given to the direct use of DNA sequencing as a detection method in its own right and to the characterization of mutations previously located by other screening techniques. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.
Comprehensive and highly practical, PCR Mutation Detection Protocols assures both seasoned and novice investigators access to the highly productive and readily reproducible PCR-based mutation detection methods, techniques that are laying the groundwork for many of today's major scientific and medical advances.

Editors and affiliations

  • Bimal D. M. Theophilus
    • 1
  • Ralph Rapley
    • 2
  1. 1.Department of HaematologyBirmingham Children’s Hospital NHS TrustBirminghamUK
  2. 2.Department of BiosciencesUniversity of HertfordshireHatfieldUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-617-8
  • Online ISBN 978-1-59259-273-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site