Malaria Methods and Protocols

Methods and Protocols

  • Denise L. Doolan

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 72)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Assessing Risk of Infection and Severity of Disease

    1. Front Matter
      Pages 1-1
    2. J. Kevin Baird, Michael J. Bangs, Jason D. Maguire, Mazie J. Barcus
      Pages 13-22
  3. Laboratory Models

    1. Front Matter
      Pages 23-23
    2. Robert E. Sinden, Geoff A. Butcher, A. L. Beetsma
      Pages 25-40
    3. Laurent Rénia, Elodie Belnoue, Irène Landau
      Pages 41-55
    4. Latifu A. Sanni, Luis F. Fonseca, Jean Langhorne
      Pages 57-76
    5. William E. Collins
      Pages 77-84
    6. William E. Collins
      Pages 85-92
  4. Diagnosis and Typing

    1. Front Matter
      Pages 93-93
    2. John C. Beier
      Pages 95-101
    3. Georges Snounou
      Pages 103-116
    4. Ingrid Felger, Hans-Peter Beck
      Pages 117-129
    5. Xin-zhuan Su, Michael T. Ferdig
      Pages 131-136
    6. Adam A. Witney, Robert M. Anthony, Daniel J. Carucci
      Pages 137-139
    7. Kyle C. McKenna, Marcelo R. S. Briones
      Pages 141-148
  5. Molecular Biology Techniques

    1. Front Matter
      Pages 149-149
    2. Tobias Spielmann
      Pages 165-175
    3. Georges Snounou, Balbir Singh
      Pages 189-203
    4. Hans-Peter Beck
      Pages 205-212
    5. Peter R. Preiser
      Pages 213-224
    6. Daniel J. Carucci, Paul Horrocks, Malcolm J. Gardner
      Pages 235-240
    7. Leda M. Cummings, Dharmendar Rathore, Thomas F. McCutchan
      Pages 241-251
    8. Dharmendar Rathore, Thomas F. McCutchan
      Pages 253-264
    9. David A. Fidock, Dharmendar Rathore, Thomas F. McCutchan
      Pages 265-275
    10. David A. Fidock, Thanh V. Nguyen, Brenda T. Beerntsen, Anthony A. James
      Pages 277-289
    11. Cecilia P. Sanchez, Martin Preuss, Michael Lanzer
      Pages 291-304
    12. Chris J. Janse, Andrew P. Waters
      Pages 305-315
    13. Vandana Thathy, Robert Ménard
      Pages 317-331
  6. Immunological Techniques

    1. Front Matter
      Pages 333-333
    2. Valentin Meraldi, Jackeline F. Romero, Giampietro Corradin
      Pages 335-345
    3. Richard C. Hedstrom, Denise L. Doolan
      Pages 347-359
    4. Isabella A. Quakyi, Jeffrey D. Ahlers
      Pages 369-383
    5. Elsa Seixas, Jean Langhorne, Stuart Quin
      Pages 385-399
    6. Chakrit Hirunpetcharat, Michael F. Good
      Pages 401-407
    7. Catherine E. M. Allsopp, Jean Langhorne
      Pages 409-421
    8. Aftab A. Ansari, Ann E. Mayne
      Pages 423-435
    9. Katrin Peter, Régine Audran, Anilza Bonelo, Giampietro Corradin, José Alejandro López
      Pages 437-444
    10. Pierre Druilhe, Hasnaa Bouharoun-Tayoun
      Pages 457-459
    11. Nicholas M. Anstey, Craig S. Boutlis, Jocelyn R. Saunders
      Pages 461-467
    12. Jocelyn R. Saunders, Mary A. Misukonis, J. Brice Weinberg, Nicholas M. Anstey
      Pages 469-474
  7. Cell Biology Techniques

    1. Front Matter
      Pages 475-475
    2. James B. Jensen
      Pages 477-488

About this book


Despite considerable scientific and medical effort over the past decades, malaria remains the most important human parasitic disease. It is responsible for up to 3 million deaths and another 300-500 million new cases each year, and is becoming resistant to the current chemoprophylactic and chemotherapeutic agents. In Malaria Methods and Protocols, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. Areas covered include clinical and laboratory diagnosis and typing, animal models, molecular biology, immunology, cell biology, vaccinology, laboratory models, and field applications. Each readily reproducible protocol has been tested, standardized, and optimized for experimental success, and includes many laboratory notes on troubleshooting, avoiding pitfalls, and interpreting results. Several of the most widely used methods are either described here in detail for the first time or have been thoroughly updated since their original publication (e.g., in vitro culture of Plasmodium parasites and in vitro growth inhibition assay).
State-of-the-art and highly practical, Malaria Methods and Protocols makes available to basic and applied researchers today's only comprehensive collection of essential laboratory methods for diagnosing malaria, characterizing the parasite, understanding the interaction between the human host and Plasmodium parasite, and developing effective preventive measures.

Editors and affiliations

  • Denise L. Doolan
    • 1
    • 2
  1. 1.Malaria Program, Naval Medical Research CenterSilver SpringUSA
  2. 2.Department of Molecular Microbiology and ImmunologySchool of Hygiene and Public Health, Johns Hopkins UniversityBaltimoreUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-823-3
  • Online ISBN 978-1-59259-271-5
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site