DNA Topoisomerase Protocols

Volume I: DNA Topology and Enzymes

  • Mary-Ann Bjornsti
  • Neil Osheroff

Part of the Methods in Molecular Biology™ book series (MIMB, volume 94)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Mary-Ann Bjornsti, Neil Osheroff
    Pages 1-8
  3. Mary-Ann Bjornsti, Maureen D. Megonigal
    Pages 9-17
  4. Albert J. Courey
    Pages 29-40
  5. Kelly L. Jordan, Devon L. Evans, David J. Hall
    Pages 41-49
  6. Giorgio Camilloni, Rodolfo Negri, Micaela Caserta, Ernesto Di Mauro
    Pages 51-60
  7. Theresa A. Shapiro, Viiu A. Klein, Paul T. Englund
    Pages 61-67
  8. Morten Isaksen, Bryan Julien, Richard Calendar, Björn H. Lindqvist
    Pages 69-74
  9. Karl Drlica, Chang-Rung Chen, Samuel Kayman
    Pages 87-98
  10. Jason D. Kahn
    Pages 109-123
  11. Anthony Maxwell, Alison J. Howells
    Pages 135-144
  12. Chang-Xi Zhu, Yuk-Ching Tse-Dinh
    Pages 145-151
  13. Hong Peng, Kenneth J. Marians
    Pages 163-169
  14. Kenneth N. Kreuzer, Sue H. Neece
    Pages 171-177
  15. Mary-Ann Bjornsti, Jolanta Fertala
    Pages 179-186
  16. Stacie J. Froelich-Ammon, Paul S. Kingma, Neil Osheroff
    Pages 199-212
  17. Lance Stewart, James J. Champoux
    Pages 223-234
  18. Scott H. Kaufmann, Phyllis A. Svingen
    Pages 253-268
  19. Piero Benedetti, Alessandra Silvestri
    Pages 269-277
  20. Back Matter
    Pages 317-327

About this book


DNA Topoisomerase Protocols, I: DNA Topology and Enzymes brings together an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, and bacterial, yeast and mammalian expression systems. A companion volume, DNA Topoisomerase Protocols, v. 2: Enzymology and Drugs, provides detailed protocols to study the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs.

DNA Topoisomerase Protocols, I: DNA Topology and Enzymes will be of immense value to the many basic scientists and clinicians who want better to understand and to exploit proficiently the wealth of recent discoveries about chromatin structure and its relation to gene expression, about DNA topoisomerases as the targets of antitumor and antibacterial agents, and about DNA repair.

Editors and affiliations

  • Mary-Ann Bjornsti
    • 1
  • Neil Osheroff
    • 2
  1. 1.St. Jude Children’s Research HospitalMemphis
  2. 2.Vanderbilt University School of MedicineNashville

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-444-0
  • Online ISBN 978-1-59259-259-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site