Vascular Disease

Molecular Biology and Gene Therapy Protocols

  • Andrew H. Baker

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 30)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Genetics in Vascular Disease

  3. Isolation of Genes Expressed in Vasular Tissue

  4. Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular Cells

  5. Molecular Analysis of MRNA Expression in Vascular Cells

  6. Molecular Methods to Study Apoptosis and Phenotypic Changes in Vascular Cells and Tissus

  7. In Vitro Methods to Express Foreign Genes in Vasular Cells

    1. Front Matter
      Pages 269-269
    2. Takamune Takahashi, Keiko Takahashi, Thomas O. Daniel
      Pages 307-314
    3. Trevor D. Littlewood, Stella A. Pelengaris
      Pages 323-341
    4. Kathryn J. Moore, Mason W. Freeman
      Pages 343-355
    5. Sarah J. George, Andrew H. Baker
      Pages 357-364
  8. In Vivo Vascular Gene Transfer Protocols

    1. Front Matter
      Pages 365-365
    2. Michael-Christopher Keogh, Daxin Chen
      Pages 385-394
    3. Mikko O. Hiltunen, Mikko P. Turunen, Seppo Ylä-Herttuala
      Pages 395-400
    4. Cathy M. Holt, Julian Gunn, Darren Lambert, David C. Cumberland, David C. Crossman
      Pages 401-415
  9. Back Matter
    Pages 437-441

About this book


In Vascular Disease: Molecular Biology and Gene Therapy Protocols, Andrew Baker and a noted panel of expert investigators describe today's most powerful molecular methods for investigating the pathogenesis of vascular disease. These detailed, easy-to-follow techniques range from methods that have been used successfully to identify specific mutations involved in cardiovascular disorders, to those for transferring genes associated with cardiovascular disease into various vascular cell types by in vitro and in vivo routes. There are methods to identify novel genes and generate full-length cDNAs, to study gene transcription and promoter activity easily and effectively, and to ascertain precisely gene expression levels within the individual cell types in different pathophysiological conditions. Accurate methods to quantify apoptosis in both cultured cells and pathological specimens are also given. Vascular Disease: Molecular Biology and Gene Therapy Protocols offers today's vascular biologist and gene therapist an unprecedented ability to study the pathogenesis of vascular disease and readily to probe the potential for gene-based therapies. Powerful and productive, the techniques presented here operate across a wide range of exciting research areas, and promise spectacular therapeutic breakthroughs in the ongoing battle against vascular disease.

Editors and affiliations

  • Andrew H. Baker
    • 1
  1. 1.Bristol Heart InstituteUniversity of BristolBristolUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-731-1
  • Online ISBN 978-1-59259-247-0
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site