Embryonic Stem Cells

Methods and Protocols

  • Kursad Turksen

Part of the Methods in Molecular Biology™ book series (MIMB, volume 185)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Pierre Savatier, Hélène Lapillonne, Ludmila Jirmanova, Luigi Vitelli, Jacques Samarut
    Pages 27-33
  3. Yong Fan, J. Richard Chaillet
    Pages 45-53
  4. David M. Adelman, M. Celeste Simon
    Pages 55-62
  5. Masahito Oyamada, Yumiko Oyamada, Tomoyuki Kaneko, Tetsuro Takamatsu
    Pages 63-69
  6. Stuart T. Fraser, Minetaro Ogawa, Satomi Nishikawa, Shin-Ichi Nishikawa
    Pages 71-81
  7. Toshiyuki Yamane, Takahiro Kunisada, Shin-Ichi Hayashi
    Pages 97-106
  8. Anna M. Wobus, Kaomei Guan, Huang-Tian Yang, Kenneth R. Boheler
    Pages 127-156
  9. Jüyrgen Hescheler, Maria Wartenberg, Bernd K. Fleischmann, Kathrin Banach, Helmut Acker, Heinrich Sauer
    Pages 169-187
  10. Tahmina Mujtaba, Mahendra S. Rao
    Pages 189-204
  11. Tammy-Claire Troy, Kursad Turksen
    Pages 229-253

About this book


Embryonic stem (ES) cells have significant potential in basic studies designed to better understand how different cells and tissues in the body are formed, as well as for generating unlimited numbers of cells for transplantation, drug delivery, and drug testing. In Embryonic Stem Cells: Methods and Protocols, Kursad Turksen and a panel of international experts describe their most productive methods for using ES cells as in vitro developmental models for many cell and tissue types. Set out in step-by-step detail by the investigators who developed them, these protocols range widely from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries. There are also advanced techniques for the generation of antibodies against very rare antigens and for the identification and characterization of proteins and protein interactions. Additional studies of the ES cell cycle and apoptosis, as well as protocols for the use of ES cells to generate diverse cell and tissue types, complete this collection of readily reproducible methods. Many of the techniques have already been shown to have tremendous utility with ES cells and their differentiated progeny.
Authoritative and state-of-the-art, this unique first collection of protocols for the study of ES cells, Embryonic Stem Cells: Methods and Protocols, will prove an invaluable resource not only for those generally interested in cell and developmental biology, but also for those actively using, or planning to use, ES cells to study fate choices and specific lineages.

Editors and affiliations

  • Kursad Turksen
    • 1
  1. 1.Ottawa Health Research InstituteOttawaCanada

Bibliographic information

  • DOI https://doi.org/10.1385/1592592414
  • Copyright Information Humana Press 2002
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-881-3
  • Online ISBN 978-1-59259-241-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book