Nuclease Methods and Protocols

  • Catherine H. Schein

Part of the Methods in Molecular Biology™ book series (MIMB, volume 160)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Characterizing Nuclease Activity

    1. Front Matter
      Pages 1-1
    2. R. Stephen Lloyd
      Pages 3-14
    3. Tania N. Gonzalez, Peter Walter
      Pages 25-36
    4. Gregor Meiss, Oleg Gimadutdinow, Peter Friedhoff, Alfred M. Pingoud
      Pages 37-48
    5. Francesco Acquati, Cinzia Nucci, Marco G. Bianchi, Tatiana Gorletta, Roberto Taramelli
      Pages 87-101
  3. Inhibitors and Activators of Nucleases

    1. Front Matter
      Pages 103-103
    2. Brittan L. Pasloske
      Pages 105-111
    3. Zeger Debyser, Peter Cherepanov, Wim Pluymers, Erik De Clercq
      Pages 139-155
    4. Richard V. Giles, David M. Tidd
      Pages 157-182
    5. Catherine Bisbal, Tamim Salehzada, Michelle Silhol, Camille Martinand, Florence Le Roy, Bernard Lebleu
      Pages 183-198
  4. Relating Nucleas Structure and Function

    1. Front Matter
      Pages 199-199
    2. Gennaro Marino, Piero Pucci, Margherita Ruoppolo
      Pages 227-236
    3. Andres Ramos, Annalisa Pastore
      Pages 237-248
    4. Kurt L. Krause, Mitchell D. Miller
      Pages 249-261
    5. Kizhake V. Soman, Catherine H. Schein, Hongyao Zhu, Werner Braun
      Pages 263-286
    6. Clark Q. Pan, Dominick V. Sinicropi, Robert A. Lazarus
      Pages 309-321
  5. Nucleases in the Clinic

    1. Front Matter
      Pages 323-323
    2. Dominick V. Sinicropi, Robert A. Lazarus
      Pages 325-333
    3. Jennifer T. Miller, Jason W. Rausch, Stuart F. J. Le Grice
      Pages 335-354
    4. Helene F. Rosenberg, Joseph B. Domachowske
      Pages 355-362
    5. James F. Riordan, Robert Shapiro
      Pages 375-385
  6. Assays Using Nucleases

  7. Back Matter
    Pages 507-525

About this book


Nucleases, the enzymes that cleave and shape nucleic acids, are key regulators of cellular metabolism. Angiogenesis and the unfolded protein response are just a few instances of important intra- and extracellular pathways regulated by nucleases. In Nuclease Methods and Protocols, researchers at the forefront of academic research and pharmaceutical drug development from four continents summarize their recent results. Their projects involve nucleases in many different areas of medicine and biology, ranging from protein structure and folding, to DNA repair, to developing cures for insidious diseases such as cystic fibrosis, AIDS, and cancer. Each chapter contains a minireview of a specific nuclease or nuclease-related theme, a discussion of why and when to use the assays described, and often, an explanation of how the assay was developed. The authors describe their most valuable methods for determining the mode of action, structure, interaction with other molecules, and physiological role of nucleases. Detailed step-by-step instructions and notes on avoiding pitfalls help ensure readily reproducible results.
Comprehensive and highly practical, Nuclease Methods and Protocols offers
both novice and experienced researchers a deeper understanding of the importance of nucleases in cell metabolism, growth control mechanisms, and, increasingly, in
diagnostics and therapeutics.

Editors and affiliations

  • Catherine H. Schein
    • 1
  1. 1.University of Texas Medical BranchGalveston

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-679-6
  • Online ISBN 978-1-59259-233-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site