Septic Shock Methods and Protocols

  • Thomas J. Evans

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 36)

Table of contents

  1. Front Matter
    Pages i-x
  2. Endotoxin

  3. Other Bacterial Products

    1. Front Matter
      Pages 57-57
    2. Manuela Roggiani, Patrick M. Schlievert
      Pages 59-66
    3. Shiranee Sriskandan
      Pages 67-79
  4. Cytokines

    1. Front Matter
      Pages 81-81
    2. Thomas J. Evans
      Pages 83-90
    3. Maarten G. Bouma, Wim A. Buurman
      Pages 91-100
    4. Daniel G. Remick, David E. Newcomb, Jon S. Friedland
      Pages 101-112
  5. Nitric Oxide and Other Reactive Nitrogen Intermediates

    1. Front Matter
      Pages 113-113
    2. Dilani K. Siriwardena, Hajime Tagori, Christoph Thiemermann
      Pages 115-131
    3. Tadeusz Malinski
      Pages 133-144
    4. Lee D. K. Buttery, Julia M. Polak
      Pages 145-157
    5. Liliana Viera, Yao Zu Ye, Joseph S. Beckman
      Pages 159-169
    6. Stuart Malcolm, Raymond Foust III, Caryn Hertkorn, Harry Ischiropoulos
      Pages 171-177
  6. Cell Culture Techniques

    1. Front Matter
      Pages 179-179
    2. Kevin A. Krown, Roger A. Sabbadini
      Pages 181-188
  7. Back Matter
    Pages 207-208

About this book


In Septic Shock Methods and Protocols, an interdisciplinary panel of expert investigators describe proven methods for investigating the often deadly mechanisms of septic shock. Drawing on insights from a wide variety of fields-ranging from molecular and cellular biology to the physiological measurement of whole animals-these experienced laboratorians detail the most important techniques for studying sepsis involving endotoxins, toxins derived from gram-positive organisms, cytokines, nitric oxide and other reactive nitrogen intermediates, and the use of primary cell cultures. They also treat a number of significant techniques that are not well described in the literature, including the Limulus endotoxin assay, the bioassay of TNF, the purification of bacterial endotoxin and superantigens, and the culture of primary human cells.

Multidisciplinary and state-of-the-art, Septic Shock Methods and Protocols offers both experimental and clinical investigators a gold-standard collection of key methods for illuminating the basic pathophysiological processes that occur following infection. Its readily reproducible methods will allow both newcomers and those with more experience to apply today's powerful techniques successfully in their work.

Editors and affiliations

  • Thomas J. Evans
    • 1
  1. 1.Imperial College School of MedicineLondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-730-4
  • Online ISBN 978-1-59259-216-6
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site