Gene Targeting Protocols

  • Eric B. Kmiec

Part of the Methods in Molecular Biology™ book series (MIMB, volume 133)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Natasha J. Caplen
    Pages 1-19
  3. Ayumi Fujita-Kusano, Yasuhiro Naito, Izumu Saito, Ichizo Kobayashi
    Pages 101-109
  4. Samuel M. Young Jr., Weidong Xiao, Richard Jude Samulski
    Pages 111-126
  5. Richard J. Bartlett, Jesica M. McCue
    Pages 127-156
  6. Juliette Fivaz, M. Chiara Bassi, Stéphane Pinaud, Larry Richman, Melanie Price, Jovan Mirkovitch
    Pages 167-182
  7. Karen M. Vasquez, John H. Wilson
    Pages 183-200
  8. Howard B. Gamper, Irina Afonina, Evgeniy Belousov, Michael W. Reed, Mikhail A. Podyminogin
    Pages 201-211
  9. Peter B. Kipp, Joyce M. Van Eck, Peter R. Beetham, Gregory D. May
    Pages 213-221
  10. Halina Sierakowska, Sudhir Agrawal, Ryszard Kole
    Pages 223-227
  11. Back Matter
    Pages 235-244

About this book


In Gene Targeting Protocols, a panel of innovative investigators present in readily reproducible detail the latest techniques for gene replacement, gene knockout, and gene repair in both animals and plants. These expert researchers review the best gene transfer vehicles (liposomes, PEI, and EPD), discuss the parameters that affect vector uptake, and detail successful methods for gene targeting (Cre-ox, and adeno and adeno-associated viruses). The methods include two fundamentally different approaches to gene targeting- one using a fusion protein to deliver a vector to a virus for possible therapeutic effect, and one entailing an episomal-based Epstein-Barr vector that can modulate the chromatin assembly process, and thus overcome a serious barrier to therapeutic gene targeting. The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may revolutionize the entire field.
Innovative and wide ranging, Gene Targeting Protocols offers today's researchers robust and proven gene-targeting techniques that are essential to understanding and regulating biological processes at the genetic level.

Editors and affiliations

  • Eric B. Kmiec
    • 1
  1. 1.University of DelawareNewark

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-360-3
  • Online ISBN 978-1-59259-215-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site