Telomeres and Telomerase

Methods and Protocols

  • John A. Double
  • Michael J. Thompson

Part of the Methods in Molecular Biology™ book series (MIMB, volume 191)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Michael C. Bibby
    Pages 1-12
  3. Harry Scherthan
    Pages 13-31
  4. Jean-Patrick Pommier, Laure Sabatier
    Pages 33-63
  5. W. Nicol Keith, Joseph Sarvesvaran, Martin Downey
    Pages 65-81
  6. Dennis S. Salonga, Kathleen D. Danenberg, Jean Grem, Ji Min Park, Peter V. Danenberg
    Pages 83-98
  7. Thomas Emrich, Sheng-Yung Chang, Gerlinde Karl, Birgit Panzinger, Chris Santini
    Pages 99-108
  8. Angelika M. Burger
    Pages 109-124
  9. Jun-ichi Nakayama, Fuyuki Ishikawa
    Pages 125-136
  10. Wade K. Aldous, Amber J. Marean, Mary J. DeHart, Katherine H. Moore
    Pages 137-146
  11. Kazuma Ohyashiki, Junko H. Ohyashiki
    Pages 159-164
  12. Henriette Gourdeau, Marsha D. Speevak, Lucie Jetté, Mario Chevrette
    Pages 173-195
  13. Imad Naasani, Takao Yamori, Takashi Tsuruo
    Pages 197-207
  14. John A. Double
    Pages 209-216
  15. Back Matter
    Pages 217-221

About this book


The discovery of telomerase expression in a majority of tumors and the recognition of its potential role in the aging process has dramatically raised its profile as a target for therapeutic intervention. In Telomeres and Telomerase: Methods and Protocols, John A. Double and Michael J. Thompson have collected a critically important series of novel and essential techniques for studying telomeres and telomerase. These readily reproducible protocols provide cutting-edge tools to identify, measure, and analyze telomeres, to determine telomerase expression at the RNA level, to determine telomerase activity, and to detect potential modifiers of this activity. The techniques for assaying telomerase activity range from standard radiological TRAP assays to nonradioactive methods, from non-PCR-based methods to techniques using real-time PCR. Written by investigators who have used the methods extensively, each protocol includes step-by-step instructions, tips on avoiding pitfalls, and invaluable notes that make all the difference to successful experimental outcomes.
Broad-ranging and highly practical, Telomeres and Telomerase: Methods and Protocols provides molecular and cell biologists, cancer researchers, and cellular pathologists with the core array of productive techniques needed today to develop telomerase inhibitors and clinically valuable diagnostic/prognostic telomerase markers.

Editors and affiliations

  • John A. Double
    • 1
  • Michael J. Thompson
    • 1
  1. 1.Cancer Research UnitUniversity of Bradford BradfordWest YorkshireUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-657-4
  • Online ISBN 978-1-59259-189-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site