Cystic Fibrosis Methods and Protocols

  • William R. Skach

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 70)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Genetics of Cystic Fibrosis

    1. Front Matter
      Pages 1-1
    2. Julian Zielenski, Isabel Aznarez, Tuncer Onay, John Tzounzouris, Danuta Markiewicz, Lap-Chee Tsui
      Pages 3-19
    3. Meera Srivastava, Ofer Eidelman, Harvey B. Pollard
      Pages 21-29
    4. Jeffrey J. Wine, Michael Dean, Damjan Glavac
      Pages 31-46
  3. CFTR structure and Function:

    1. Front Matter
      Pages 47-47
    2. Structure, Gating, and Regulation

      1. Allan Powe, Zhen Zhou, Tzyh-Chang Hwang, Georg Nagel
        Pages 67-98
      2. Tang Zhu, Deborah A. R. Hinkson, David Dahan, Alexandra Evagelidis, John W. Hanrahan
        Pages 99-109
      3. Ashvani K. Singh, Sangeeta Singh, Daniel C. Devor, Raymond A. Frizzell, Willy van Driessche, Robert J. Bridges
        Pages 129-142
      4. Ilana Kogan, Mohabir Ramjeesingh, Canhui Li, Christine E. Bear
        Pages 143-157
      5. Alan S. Verkman, Sujatha Jayaraman
        Pages 187-196
    3. Expression, Folding, and Degradation

      1. Mohamed Benharouga, Manu Sharma, Gergely L. Lukacs
        Pages 229-243
      2. Geoffrey C. Meacham, Douglas M. Cyr
        Pages 245-256
      3. Yimao Zhang, Susan Michaelis, Jeffrey L. Brodsky
        Pages 257-265
      4. Marybeth Howard, William J. Welch
        Pages 267-275
      5. Michael J. Corboy, Philip J. Thomas, W. Christian Wigley
        Pages 277-294
      6. Jon Oberdorf, William R. Skach
        Pages 295-310
      7. Rhesa D. Stidham, W. Christian Wigley, Philip J. Thomas
        Pages 311-322
      8. Kelly Weixel, Neil A. Bradbury
        Pages 323-340
    4. Regulatory Complexes

      1. Scott H. Donaldson, Elaine G. Poligone, M. Jackson Stutts
        Pages 343-364
      2. Viswanathan Raghuram, Kenneth R. Hallows, J. Kevin Foskett
        Pages 365-381
      3. Peter J. Mohler, Patricia L. Kultgen, M. Jackson Stutts, Sharon L. Milgram
        Pages 383-394
      4. Marie E. Egan
        Pages 395-406
  4. Pathophysiology of Cystic Fibrosis

    1. Front Matter
      Pages 407-407
    2. Jay B. Hilliard, Michael W. Konstan, Pamela B. Davis
      Pages 409-431
    3. Scott D. Sagel, Elaine B. Dowell, Frank J. Accurso
      Pages 433-446
    4. Alexander M. Cole, Tomas Ganz
      Pages 447-464
    5. Ruth Bryan, Alice Prince
      Pages 465-478
    6. Animal Models of Cystic Fibrosis

      1. James F. Chmiel, Michael W. Konstan, Melvin Berger
        Pages 495-515
      2. Steven D. Freedman, Paola G. Blanco, Julie C. Shea, Juan G. Alvarez
        Pages 517-524
      3. Barbara R. Grubb
        Pages 525-535
      4. Mohammed Filali, Yulong Zhang, Teresa C. Ritchie, John F. Engelhardt
        Pages 537-550
      5. Jean-Marie Zahm, Sonia Baconnais, Gérard Balossier, Edith Puchelle
        Pages 563-572
  5. Novel Therapeutic Approaches for Cystic Fibrosis

    1. Front Matter
      Pages 573-573
    2. Kimberly V. Curlee, Eric J. Sorscher
      Pages 575-584
    3. John Marshall, Seng H. Cheng
      Pages 585-598
    4. Terence R. Flotte, Isabel Virella-Lowell, Kye A. Chesnut
      Pages 599-608
  6. Back Matter
    Pages 609-631

About this book


Since the cloning of the cystic fibrosis transmembrane conductance regulator (CFTR) nearly a decade ago, cystic fibrosis researchers, clinicians, and patients have come to rely increasingly on a diverse array of fundamental techniques to understand the molecular basis of this complex disease. Cystic Fibrosis Methods and Protocols consolidates a broad range of detailed and readily reproducible in vitro, cellular, and whole animal laboratory protocols into an indispensable resource. From electrophysiology and cell biology, to animal models and gene therapy, this comprehensive set of methods provides the step-by-step instructions needed for investigators to incorporate new approaches into their research programs. Specific protocols describe new techniques for diagnosis, in vitro methods for the expression and functional analysis of CFTR, novel biochemical and cellular systems to determine how mutations subvert CFTR function, and in vivo protocols to examine how CFTR dysfunction produces multisystem pathology in human and animal models.
Comprehensive, multidisciplinary, and highly practical, Cystic Fibrosis Methods and Protocols makes accessible to today's cystic fibrosis investigator the powerful new scientific techniques required to investigate the basic science of the disease and to translate this into effective clinical solutions.

Editors and affiliations

  • William R. Skach
    • 1
  1. 1.Division of Molecular MedicineOregon Health Sciences UniversityPortland

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-897-4
  • Online ISBN 978-1-59259-187-9
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site