Neural Stem Cells: Methods and Protocols

  • Tanja Zigova
  • Paul R. Sanberg
  • Juan R. Sanchez-Ramos

Part of the Methods in Molecular Biology™ book series (MIMB, volume 198)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Isolation and Culture of NSCs

    1. Front Matter
      Pages 1-1
    2. Eric D. Lay well, Valery G. Kukekov, Oleg Suslov, Tong Zheng, Dennis A. Steindler
      Pages 15-26
    3. Yuan Y. Wu, Tahmina Mujtaba, Mahendra S. Rao
      Pages 29-39
    4. Susan C. Barnett, A. Jane Roskams
      Pages 41-47
    5. Seung U. Kim, Eiji Nakagawa, Kozo Hatori, Atsushi Nagai, Myung A. Lee, Jung H. Bang
      Pages 55-64
    6. Lamya S. Shihabuddin
      Pages 67-76
    7. Erin Lavik, Yang D. Teng, Evan Snyder, Robert Langer
      Pages 89-96
  3. Characterization of NSCs in VITRO

    1. Front Matter
      Pages 99-99
    2. Cellular Techniques

      1. Takao Takahashi, Verne S. Caviness Jr., Pradeep G. Bhide
        Pages 101-112
      2. Angelo L. Vescovi, Rossella Galli, Angela Gritti
        Pages 115-122
      3. Mark P. Mattson, Peisu Zhang, Weiming Fu
        Pages 125-135
      4. Karen R. Prowse
        Pages 137-146
      5. Bela Kosaras, Evan Snyder
        Pages 157-174
    3. Electrophysiological Techniques

    4. Molecular Techniques

      1. Barbara A. Tate, Rachel L. Ostroff
        Pages 189-194
      2. Theresa E. Gratsch
        Pages 213-220
      3. Terence J. Van Raay, Michael R. Stark
        Pages 223-231
      4. Luciano Conti, Elena Cattaneo
        Pages 233-243
      5. Simonetta Sipione, Elena Cattaneo
        Pages 245-261
  4. Utilization/Characterization of NSCs in Vivo

    1. Front Matter
      Pages 263-263
    2. Endogenous Pools of Stem/Progenitor Cells

      1. Sanjay S. P. Magavi, Jeffrey D. Macklis
        Pages 283-289
      2. Sanjay S. P. Magavi, Jeffrey D. Macklis
        Pages 291-297
      3. Lisa A. Catapano, Sanjay S. P. Magavi, Jeffrey D. Macklis
        Pages 299-303
    3. Transplantation

      1. Scott R. Whittemore, Y. Ping Zhang, Christopher B. Shields, Dante J. Morassutti, David S.K. Magnuson
        Pages 319-324
      2. Alison E. Willing, Svitlana Garbuzova-Davis, Paul R. Sanberg, Samuel Saporta
        Pages 357-373
  5. Back Matter
    Pages 375-381

About this book


Over the last decade, advances in neural stem cell research have raised the hope that one day cellular therapy will not only replace missing neurons, but also replenish absent chemical signals, metabolites, enzymes, neurotransmitters, or other missing or defective components from the diseased or injured brain. In Neural Stem Cells: Methods and Protocols, internationally recognized experts from academic, clinical, and pharmaceutical laboratories describe in great detail the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These readily reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. Additional techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. The protocols range from the simplest methods of isolation and characterization of neural cell properties to such very sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics. Each is written by an investigator who has used the method extensively, and includes step-by-step instructions, tips on avoiding pitfalls, and invaluable notes that make all the difference to successful experimental outcomes.
Comprehensive and easy-to-follow, Neural Stem Cells: Methods and Protocols provides a powerful synthesis of today's key in vitro and in vivo techniques to identify and characterize neural stem cells for research and for developing experimental therapeutics.

Editors and affiliations

  • Tanja Zigova
    • 1
  • Paul R. Sanberg
    • 1
  • Juan R. Sanchez-Ramos
    • 1
  1. 1.Department of NeurosurgeryUniversity of South Florida College of MedicineTampa

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-964-3
  • Online ISBN 978-1-59259-186-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site