Table of contents

  1. Front Matter
    Pages i-xvii
  2. Labeling DNA Breaks Using Terminal Transferase (TUNEL Assay)

  3. Labeling DNA Breaks Using DNA Polymerase I or its Klenow Fragment

    1. Front Matter
      Pages 79-79
    2. Katherine A. Wood
      Pages 109-119
  4. Labeling DNA Breaks Using Ligase

    1. Front Matter
      Pages 131-131
    2. Peter J. Hornsby, Vladimir V. Didenko
      Pages 133-141
    3. Vladimir V. Didenko, Hop Ngo, David S. Baskin
      Pages 153-159
  5. Detection of DNA Breaks in Agarose Trapped Cells: Comet Assay and Related Techniques

    1. Front Matter
      Pages 161-161
    2. Andrew R. Collins
      Pages 163-177
    3. Peggy L. Olive
      Pages 179-194

About this book

Introduction

Detection and analysis of DNA damage is critically important in the study of the widest variety of biological phenomena, ranging from apoptosis and aging to heart diseases and anticancer drug development. In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and show how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. In-depth protocols for single-cell electrophoresis (comet assay) are presented, as well as those for the detection of indirect markers of DNA damage and modified bases. Technical reviews discuss specificity, sensitivity, advantages, and limitations of the techniques described, and compare them with alternative approaches.
State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying not only the in situ detection of DNA damage but also the labeling of apoptotic cells.

Keywords

DNA FISH Fluoreszenz in situ-Hybridisierung Ligation Termination Translation electron microscopy microscopy reproducible techniques

Editors and affiliations

  • Vladimir V. Didenko
    • 1
  1. 1.Baylor College of MedicineHouston

Bibliographic information

  • DOI https://doi.org/10.1385/1592591795
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Book Archive
  • Print ISBN 978-0-89603-952-0
  • Online ISBN 978-1-59259-179-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book