PCR Cloning Protocols

  • Bing-Yuan Chen
  • Harry W. Janes

Part of the Methods in Molecular Biology™ book series (MIMB, volume 192)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Performing and Optimizing PCR

    1. Front Matter
      Pages 1-108
    2. Lori A. Kolmodin, David E. Birch
      Pages 3-18
    3. Bing-Yuan Chen, Harry W. Janes, Steve Chen
      Pages 19-29
    4. Volker Thiel, Jens Herold, Stuart G. Siddell
      Pages 59-66
    5. Abebe Akalu, Juergen K. V. Reichardt
      Pages 67-74
    6. Alain Moreau, Da Shen Wang, Steve Forget, Colette Duez, Jean Dusart
      Pages 75-80
    7. Haruhiko Ohashi
      Pages 91-97
  3. Cloning PCR Products

    1. Front Matter
      Pages 109-163
    2. Baotai Guo, Yuping Bi
      Pages 111-119
    3. Brett A. Neilan, Daniel Tillett
      Pages 125-132
    4. József Gál, Miklós Kálmán
      Pages 141-151
    5. Tamara S. Gritsun, Michael V. Mikhailov, Ernest A. Gould
      Pages 153-163
  4. Mutagenesis and Recombination

    1. Front Matter
      Pages 165-245
    2. Sailen Barik
      Pages 189-196
    3. Guowei Fang, Barbara Weiser, Aloise Visosky, Timothy Moran, Harold Burger
      Pages 197-206
    4. Dario E. Lehoux, Roger C. Levesque
      Pages 225-234
    5. Anna Marie Aguinaldo, Frances Arnold
      Pages 235-239
    6. Donghak Kim, F. Peter Guengerich
      Pages 241-245
  5. Cloning Unknown Neighboring DNA

    1. Front Matter
      Pages 247-350
    2. Eric Ka-Wai Hui, Po-Ching Wang, Szecheng J. Lo
      Pages 249-274
    3. James A. L. Fenton, Guy Pratt, Gareth J. Morgan
      Pages 275-284
    4. Sheng-He Huang
      Pages 293-299
    5. Ambrose Y. Jong, Anna T′ang, De-Pei Liu, Sheng-He Huang
      Pages 301-307
    6. Vincent J. J. Martin, William W. Mohn
      Pages 315-323
  6. Library Construction and Screening

About this book


In the post-genomic era, PCR has become the method of choice not only for cloning existing genes, but also for generating a wide array of novel genes by mutagenesis and/or recombination within the genes of interest. PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long-distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination and to clone the challenging uncharacterized DNA flanking a known DNA fragment. Powerful applications of PCR in library construction and sublibrary generation and screening are also presented.
Authoritative and up-to-date, PCR Cloning Protocols, Second Edition, constitutes a gold-standard collection of the fastest, simplest, and most popular methods for isolating genes from all biological samples and creating novel genes from them by mutagenesis/recombination-essential methods for today's study of functional genomics, gene expression, protein structure-function relationships, protein engineering, and molecular evolution.


Bur DNA Mutation PCR genes hybridization recombination transcription

Editors and affiliations

  • Bing-Yuan Chen
    • 1
  • Harry W. Janes
    • 1
  1. 1.Department of Plant ScienceRutgers UniversityNew Brunswick

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-969-8
  • Online ISBN 978-1-59259-177-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site