The Protein Protocols Handbook

  • John M. Walker

Table of contents

  1. Front Matter
    Pages i-xxiv
  2. Quantitation of Proteins

    1. Front Matter
      Pages 1-1
    2. Alastair Aitken, Michèle P. Learmonth
      Pages 3-6
    3. Jakob H. Waterborg
      Pages 7-9
    4. Nicholas J. Kruger
      Pages 15-21
    5. Robert E. Akins, Rocky S. Tuan
      Pages 23-30
    6. Scott A. Boerner, Yean Kit Lee, Scott H. Kaufmann, Keith C. Bible
      Pages 31-40
    7. Alastair Aitken, Michèle Learmonth
      Pages 41-44
    8. Thomas D. Friedrich, F. Andrew Ray, Judith A. Laffin, John M. Lehman
      Pages 45-50
    9. Douglas D. Root, Kuan Wang
      Pages 51-54
  3. Electrophoresis of Proteins and Peptides and Detection in Gels

    1. Front Matter
      Pages 55-55
    2. Elisabetta Gianazza
      Pages 169-180
    3. Mustafa Ünlü, Jonathan Minden
      Pages 185-196
    4. Peter F. Lemkin, Gregory C. Thornwall
      Pages 197-214
    5. Barbara Magi, Luca Bini, Sabrina Liberatori, Roberto Raggiaschi, Vitaliano Pallini
      Pages 215-229
    6. Bryan John Smith
      Pages 237-242
    7. Joan-Ramon Daban, Salvador Bartolomé, Antonio Bermúdez, F. Javier Alba
      Pages 243-249
    8. Carlos Fernandez-Patron
      Pages 251-258
    9. Jung-Kap Choi, Hee-Youn Hong, Gyurng-Soo Yoo
      Pages 259-263
    10. Paul Jenö, Martin Horst
      Pages 299-305
    11. Antonella Circolo, Sunita Gulati
      Pages 307-314
  4. Blotting and Detection Methods

    1. Front Matter
      Pages 315-315
    2. Mark Page, Robin Thorpe
      Pages 317-319
    3. Patricia Gravel
      Pages 321-334
    4. John M. Walker
      Pages 335-336
    5. G. Brian Wisdom
      Pages 343-344
    6. G. Brian Wisdom
      Pages 345-346
    7. G. Brian Wisdom
      Pages 349-350

About this book


From Reviews of the First Edition
"...a must for researchers in protein chemistry and especially those with less experience..."
-Doody's Health Sciences Book Review Journal
"...definitely an indispensable benchtop handbook...highly recommended for all levels of research
within the field."-Bioseparation
"...a very useful tool for daily work." -Nahrung-Food

Since publication of the bestselling first edition of John Walker's widely acclaimed Protein Protocols Handbook in 1996, there have been many highly significant developments in protein biochemistry. This greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles. The new chapters cover many rapidly developing areas, particularly the application of mass spectrometry in protein characterization, as well as the now well-established 2-D PAGE technique in proteomics. The section on glycoprotein analysis has also been significantly expanded, and methods for the production of single-chain and phage-displayed antibodies have been added to the section on antibodies.
Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The authors are commonly the techniques' originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.
Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Second Edition is today's indispensable benchtop manual and guide-not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and still more robust results.


biology chemistry molecular biology nucleic acid protein proteins

Editors and affiliations

  • John M. Walker
    • 1
  1. 1.University of HertfordshireHatfieldUK

Bibliographic information