Dendritic Cell Protocols

  • Stephen P. Robinson
  • Andrew J. Stagg
  • Stella C. Knight

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 64)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Isolation of Dendritic Cells from Animal Tissues

    1. Front Matter
      Pages 1-1
    2. Andrew J. Stagg, Fiona Burke, Suzanne Hill, Stella C. Knight
      Pages 9-22
    3. Fabienne Anjuère, Carlos ArdavÍn
      Pages 23-28
    4. Gordon MacPherson, Michelle Wykes, Fang-Ping Huang, Chris Jenkins
      Pages 29-41
    5. Franz Koch, Eckhart Kämpgen, Gerold Schuler, Nikolaus Romani
      Pages 43-62
    6. Philip A. Stumbles, Jennifer Thomas, Patrick G. Holt
      Pages 73-83
    7. Lina Lu, Maureen L. Drakes, Angus W. Thomson
      Pages 85-95
  3. Isolation and Characterization of Human Dendritic Cells

    1. Front Matter
      Pages 97-97
    2. Steven Patterson, Aaron Rae, Heather Donaghy
      Pages 111-120
    3. Paul U. Cameron, Gianna Stent
      Pages 121-136
    4. Christoph U. Brand, Nikhil Yawalkar, Robert E. Hunger, Lasse R. Braathen
      Pages 137-144
    5. Cornelia D. Richters, Matthias J. Hoekstra, John S. du Pont, Robert W. Kreis, Eduard W. A. Kamperdijk
      Pages 145-153
    6. Michel M. van den Heuvel, Carin E. G. Havenith, Robert H. J. Beelen
      Pages 163-173
    7. Allison R. Pettit, Lois Cavanagh, Ranjeny Thomas
      Pages 175-187
  4. Derivation of Animal Dendritic Cells from Progenitor Cells

    1. Front Matter
      Pages 189-189
    2. Timothy Powell, James R. Major, Gordon MacPherson
      Pages 199-205
    3. Karen Lucas, Dolores Saunders, Li Wu
      Pages 207-216
  5. Generation of Murine Dendritic Cell Lines

    1. Front Matter
      Pages 217-217
    2. Stefania Citterio, Maria Rescigno, Maria Foti, Francesca Granucci, Malgosia Matyszak, Giampiero Girolomoni et al.
      Pages 219-230
    3. Akira Takashima, Paul R. Bergstresser
      Pages 243-254
  6. Generation of Human Dendritic Cells from Progenitor Cells

    1. Front Matter
      Pages 255-255
    2. Christophe Caux, Bertrand Dubois
      Pages 257-273
    3. Winfried F. Pickl, Otto Majdic, Walter Knapp
      Pages 283-296
  7. Aspects of Dendritic Cell Function

    1. Front Matter
      Pages 305-305
    2. Walter Luini, Giovanna D’Amico, Sergio Bernasconi, Paola Allavena, Alberto Mantovani, Silvano Sozzani
      Pages 307-312
    3. K. Alun Brown
      Pages 313-323
    4. Giancarlo Bianchi, Giovanna D’Amico, Silvano Sozzani, Alberto Mantovani, Paola Allavena
      Pages 325-330
    5. Marie Cumberbatch, Rebecca J. Dearman, Ian Kimber
      Pages 331-346
    6. Kenjiro Matsuno, Taichi Ezaki
      Pages 347-368
    7. Christophe Caux, Bertrand Dubois
      Pages 369-376
    8. Neil A. Fanger, Paul M. Guyre, Robert F. Graziano
      Pages 377-386
    9. Monique J. Kleijmeer, George Posthuma, Hans J. Geuze
      Pages 387-411
    10. Wenru Song, Ronald G. Crystal
      Pages 429-439
    11. Xiaoli Huang, Zheng Fan, Lian Zheng, Charles R. Rinaldo Jr.
      Pages 441-453
  8. Back Matter
    Pages 455-470

About this book


It is now apparent that dendritic cells not only play important roles in the body's immune system through their complex interactions with T cells, B cells, and other cell types, but also possess distinct functional attributes that enable them to assume different roles in that system. In Dendritic Cell Protocols, Stephen P. Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these so-called "veiled" cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide variety of tissues or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. Variant methods that have been successful in other laboratories have been included to expand experimental possibilities. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeutics.

State-of-the-art and highly practical, Dendritic Cell Protocols provides both experienced and novice investigators with powerful tools to illuminate the complex biology of these foundation cells-cells that shape the evolution of acquired immune responses, play important roles in innate immunity, and promise the development of powerful new immunotherapeutics.

Editors and affiliations

  • Stephen P. Robinson
    • 1
  • Andrew J. Stagg
    • 2
  • Stella C. Knight
    • 3
  1. 1.Department of HematologyUniversity College Hospital LondonUK
  2. 2.Antigen Presentation Research GroupImperial College School of Medicine, at Northwick Park HospitalHarrowUK
  3. 3.Professor of ImmunopathologyImperial College School of MedicineLondon

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-584-3
  • Online ISBN 978-1-59259-150-3
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site