Mycobacterium tuberculosis Protocols

  • Tanya Parish
  • Neil G. Stoker

Part of the Methods in Molecular Medicine book series (MIMM, volume 54)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Nicola Casali, Sabine Ehrt
    Pages 1-17
  3. Paul D. van Helden, Thomas C. Victor, Robin M. Warren, Eileen G. van Helden
    Pages 19-30
  4. Irene M. Monahan, Joseph A. Mangan, Philip D. Butcher
    Pages 31-42
  5. Stoyan S. Bardarov, Svetoslav S. Bardarov Jr., William R. Jacobs Jr.
    Pages 43-57
  6. Mary Jackson, Luis Reinaldo Camacho, Brigitte Gicquel, Christophe Guilhot
    Pages 59-75
  7. Bhavna G. Gordhan, Tanya Parish
    Pages 77-92
  8. Farahnaz Movahedzadeh, Jorge A. Gonzalez-Y-Merchand, Robert A. Cox
    Pages 105-124
  9. Rory P. Cooney, Natalie J. Garton, Michael R. Barer
    Pages 125-143
  10. Dick van Soolingen, Petra E. W. de Haas, Kristin Kremer
    Pages 165-203
  11. Ida Rosenkrands, Peter Andersen
    Pages 205-215
  12. Mamadou Daffé, Marie-Antoinette Lanée
    Pages 217-227
  13. Richard A. Slayden, Clifton E. Barry 3rd
    Pages 229-245
  14. Pauline T. Lukey, Elizabeth U. Hooker
    Pages 271-280
  15. Riccardo Manganelli, Sanjay Tyagi, Issar Smith
    Pages 295-310
  16. Joanna C. Betts, Marjorie A. Smith
    Pages 315-334
  17. Michael Wilson, Martin Voskuil, Dirk Schnappinger, Gary K. Schoolnik
    Pages 335-357
  18. Kristin Kremer, Tridia van der Laan, Dick van Soolingens
    Pages 359-365
  19. Heidi Alderton, Debbie Smith
    Pages 367-383
  20. Back Matter
    Pages 385-403

About this book


With one-third of the world's population infected with Mycobacterium tuberculosis, over two million people a year dying from tuberculosis, and the appearance of multidrug-resistant strains, the need to understand the biology of M. tuberculosis, and so to develop new interventions, has become acute. In Mycobacterium tuberculosis Protocols, leading investigators with extensive practical knowledge and experience describe their best methods for studying this dangerous pathogen. Packed with step-by-step instructions to ensure successful results, these methods range from basic handling techniques to the application of functional genomics. These molecular techniques are suitable for research in genetics, biochemistry, microbiology, cell biology, epidemiology, and diagnostics, and are at the forefront of biological research as a whole, as well as in focused M. tuberculosis research. Highlights include methods for the basic safety and culture of M. tuberculosis, fractionation of the bacterium (nucleic acids, lipids, culture filtrate, and capsule), the analysis of gene expression (start-site mapping, real-time PCR, microarrays, and proteomics), the growth of the bacterium in macrophages and low oxygen, cytological analysis of the bacteria, and diagnostics. Highly practical and accessible, Mycobacterium tuberculosis Protocols utilizes advanced functional genomics and mutagenesis methodologies to provide both experimental and clinical investigators all the powerful techniques needed to illuminate the molecular biology of tuberculosis and its interactions with host cells, and so drive work on the wide variety of emerging therapeutic opportunities.

Editors and affiliations

  • Tanya Parish
    • 1
  • Neil G. Stoker
    • 2
  1. 1.St. Bartholomew’s and Royal London School of Medicine and DentistryLondonUK
  2. 2.London School of Hygiene and Tropical MedicineLondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-776-2
  • Online ISBN 978-1-59259-147-3
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site