Hematopoietic Stem Cell Protocols

  • Christopher A. Klug
  • Craig T. Jordan

Part of the Methods in Molecular Medicine book series (MIMM, volume 63)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Elaine Dzierzak, Marella de Bruijn
    Pages 1-14
  3. Terry E. Thomas, Sara J. Abraham, Peter M. Lansdorp
    Pages 29-57
  4. Michael J. Reitsma, Brenda R. Lee, Nobuko Uchida
    Pages 59-77
  5. Makio Ogawa, Anne G. Livingston
    Pages 113-122
  6. Cindy L. Miller, Connie J. Eaves
    Pages 123-141
  7. Gerald de Haan, Rob Ploemacher
    Pages 143-151
  8. Ernest A. McCulloch
    Pages 153-160
  9. Libuse Jerabek, Irving L. Weissman
    Pages 161-165
  10. Stephen J. Szilvassy, Franck E. Nicolini, Connie J. Eaves, Cindy L. Miller
    Pages 167-187
  11. Cindy L. Miller, Julie Audet, Connie J. Eaves
    Pages 189-208
  12. Gordon M. Keller, Saiphone Webb, Marion Kennedy
    Pages 209-230
  13. Christian P. Kalberer, Jennifer Antonchuk, R. Keith Humphries
    Pages 231-242
  14. Claudiu V. Cotta, C. Weissman, Irving L. Weissman, Christopher A. Klug
    Pages 243-252
  15. Back Matter
    Pages 327-332

About this book


Hematopoietic stem cells have an enormous clinical importance for bone marrow transplantation, hematopoietic gene therapy, solid organ transplantation, and somatic tissue regeneration. In Hematopoietic Stem Cell Protocols, a group of leading stem-cell investigators describe in detail the key laboratory methods for investigating this critically important cell type. Varied in scope, and richly detailed with step-by-step instructions and notes on the pitfalls to be avoided, these readily reproducible protocols apply hematopoietic stem cells isolated from mouse and human tissues of both embryonic and adult origin. The many approaches described employ not only molecular and cell biology techniques, but also animal model systems, and include hematopoietic stem cell expansion protocols and methods for the purification and genetic modification of stem cells. Many of the protocols are contributed by those researchers who first developed and introduced the procedures to the scientific community.
Comprehensive and state-of-the-art, Hematopoietic Stem Cell Protocols offers both novice and experienced investigators alike an invaluable compendium of highly practical techniques for studying a wide variety of stem cell topics, including blood formation, blood-based disease, and such blood cancers as leukemia.

Editors and affiliations

  • Christopher A. Klug
    • 1
  • Craig T. Jordan
    • 2
  1. 1.University of Alabama-BirminghamBirminghamAL
  2. 2.University of KentuckyLexingtonKY

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-812-7
  • Online ISBN 978-1-59259-140-4
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site