Overview
- Editors:
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Christopher A. Klug
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University of Alabama-Birmingham, Birmingham, AL
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Craig T. Jordan
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University of Kentucky, Lexington, KY
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Table of contents (20 protocols)
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- Elaine Dzierzak, Marella de Bruijn
Pages 1-14
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- Terry E. Thomas, Sara J. Abraham, Peter M. Lansdorp
Pages 29-57
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- Michael J. Reitsma, Brenda R. Lee, Nobuko Uchida
Pages 59-77
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- Gillian B. Bradford, Ivan Bertoncello
Pages 79-92
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- Edward F. Srour, Craig T. Jordan
Pages 93-111
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- Makio Ogawa, Anne G. Livingston
Pages 113-122
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- Cindy L. Miller, Connie J. Eaves
Pages 123-141
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- Gerald de Haan, Rob Ploemacher
Pages 143-151
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- Libuse Jerabek, Irving L. Weissman
Pages 161-165
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- Stephen J. Szilvassy, Franck E. Nicolini, Connie J. Eaves, Cindy L. Miller
Pages 167-187
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- Cindy L. Miller, Julie Audet, Connie J. Eaves
Pages 189-208
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- Gordon M. Keller, Saiphone Webb, Marion Kennedy
Pages 209-230
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- Christian P. Kalberer, Jennifer Antonchuk, R. Keith Humphries
Pages 231-242
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- Claudiu V. Cotta, C. Weissman, Irving L. Weissman, Christopher A. Klug
Pages 243-252
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About this book
The ability to highly purify and characterize hematopoietic stem cells (HSC) from mice and humans has opened up an exceedingly rich field of basic science research with enormous clinical potential. Many of the techniques used in st- ies of HSC biology have become more standardized over the last several years, which makes it possible to compile a set of methods that can be used by both seasoned investigators and novices in the stem cell field. We have attempted to be as comprehensive as possible and yet focus on what we perceive to be the most widely used approaches for studies of murine and human HSC. This first edition of Hematopoietic Stem Cell Protocols will therefore have some obvious omissions that were dictated by contemporary circumstances. It is our hope that readers will feel free to contribute their personal suggestions for further chapters as well as on how existing chapters can be improved for future editions. We certainly expect that old approaches will be refined, new assays will be developed, and other animal model and vector systems will be described that will become the new gold standards for future work. Our s- cere thanks goes out to all of the contributors and to those in the stem cell field that have enlarged our thinking and provided new tools to further understand this fascinating cell type.
Reviews
"Knowledge of hematopoietic stem cells is crucial in the development of clinical medicine, especially hematopoietic stem cell transplantation, solid organ transplantation, tissue engineering for the treatment of damaged tissue. Research in the area has made major advance in the past two decades. Various technologies were produced and contributed to the development. This book describes detailed techniques in each experiment as if authors are teaching to the investigators by their side. Each section also contains notes and introductions that explain practical tips, historical and logical aspects of those experiments. This book covers current important methodologies in hematopoietic stem cell research and helps investigators to perform correct techniques excellently. "- Doody's Health Sciences Book Review Journal. Numerical Score: 91 - 4 Stars!
"The book presents intimate knowledge of stem cell technology and useful practical guide through laboratory protocols. Despite detailed step by step protocols with plenty of valuable pieces of information about critical assay points, book offers even a comprehensive biological and molecular background of HSC. Essential for novices and inspiring for experienced investigators, Hematopoietic Stem Cell Protocols, will not have a fate of book forgotten at the upper bookcase shelf but can accomplish the idea of information source easily reached at your bench." The book is well referenced, and readers will easily be able to locate primary literature for in-depth reading on specific topics. The figures and illustrations are extremely useful in summarizing complex topics that are discussed." -Neoplasma
"Comprehensive and state-of-the-art, Hematopoietic Stem Cell Protocols offers both novice and experienced investigators alike an invaluable compendium of highly practical techniques for studying a wide variety of stem cell topics, including blood formation, blood-based disease, and such blood cancers as leukemia." -Clinical Laboratory
Editors and Affiliations
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University of Alabama-Birmingham, Birmingham, AL
Christopher A. Klug
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University of Kentucky, Lexington, KY
Craig T. Jordan
