Muscular Dystrophy

Methods Protocols

  • Katharine M.D. Bushby
  • Louise V.B. Anderson

Part of the Methods in Molecular Medicine book series (MIMM, volume 43)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Background

    1. Front Matter
      Pages 1-1
    2. Katharine M. D. Bushby, Louise V. B. Anderson
      Pages 3-8
    3. David Gardner-Medwin
      Pages 9-13
    4. Margaret A. Johnson
      Pages 15-30
    5. Mayana Zatz, Mariz Vainzof, Maria Rita Passos-Bueno
      Pages 31-49
  3. The molecular approach

    1. Front Matter
      Pages 51-51
    2. Johan T. den Dunnen
      Pages 85-109
    3. Anne Helbling-Leclerc, Pascale Guicheney
      Pages 199-218
    4. Frederica Piccolo, C. de Toma, Marc Jeanpierre
      Pages 219-226
    5. Carsten G. Bönnemann, Louis M. Kunkel
      Pages 227-255
    6. Vincenzo Nigro
      Pages 257-272
    7. Isabelle Richard, Jacques Beckmann
      Pages 273-287
    8. Rumaisa Bashir, Ruth Harrison, Robert H. Brown Jr.
      Pages 289-303
    9. Silvére M. van der Maarel, Egbert Bakker, Rune R. Frants
      Pages 305-316
  4. Protein Analysis in the Muscular Dystrophies

    1. Front Matter
      Pages 317-317
    2. Louise V. B. Anderson
      Pages 319-324
    3. Caroline A. Sewry, Qui Lu
      Pages 325-338
    4. Margaret A. Johnson
      Pages 339-367
    5. Eric P. Hoffman, Mark Evans
      Pages 387-391
    6. Mark M. Rich, Rita J. Balice-Gordon, Sita Reddy
      Pages 395-408
    7. Matthew G. Dunckley, George Dickson
      Pages 409-434
  5. Back Matter
    Pages 435-457

About this book


With the molecular mechanisms underlying the various forms of muscular dystrophy now rapidly clarifying, precise diagnosis has become a reality, and even a requirement in clinical practice. In Muscular Dystrophy: Methods and Protocols, Katherine Bushby and Louise Anderson have assembled an outstanding collection of key techniques for the analysis of DNA and protein from patients suspected to suffer from muscular dystrophy. Each method is highly detailed to ensure success and is presented by a hands-on expert who uses it on a day-to-day basis. The various DNA techniques focus on both the X-linked muscular dystrophies and the autosomal recessive muscular dystrophies. The protein methods include expression analysis, multiplex western blot analysis, immunocytochemical analysis, and reviews of immunological reagants and of amplification systems. Also discussed are the use of animal models to understand human muscular dystrophy and the available options for gene-based therapy.
Comprehensive and highly practical, Muscular Dystrophy: Methods and Protocols offers today‚s diagnostic laboratories, basic and medical researchers, and active clinicians an authoritative collection of tools that will serve as exacting diagnostic tools as well as greatly empowering research on the novel therapeutics now beginning to emerge.

Editors and affiliations

  • Katharine M.D. Bushby
    • 1
  • Louise V.B. Anderson
    • 2
  1. 1.Department of Human Genetics, School of Biochemistry and GeneticsUniversity of Newcastle upon TyneNewcastle upon TyneUK
  2. 2.Department of NeurobiologyMedical School, University of Newcastle upon TyneNewcastle upon TyneUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-695-6
  • Online ISBN 978-1-59259-138-1
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site