Metastasis Research Protocols

Volume II: Analysis of Cell Behavior In Vitro and In Vivo

  • Susan A. Brooks
  • Udo Schumacher

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 58)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Selection/Segregation Cell Populations for in Vitro and in Vivo Assays of Metastatic Behavior

    1. Front Matter
      Pages 1-1
    2. Derek Davies
      Pages 3-15
    3. Catherine Clarke, Susan Davies
      Pages 17-23
  3. In Vitro Assays of Metastatic Behavior

    1. Front Matter
      Pages 31-31
    2. Tom Boterberg, Marc E. Bracke, Erik A. Bruyneel, Marc M. Mareel
      Pages 33-45
    3. Nicholas S. Brown, Roy Bicknell
      Pages 47-54
    4. Yasufumi Niinaka, Arayo Haga, Avraham Raz
      Pages 55-60
    5. Debbie M., Susan A. Brooks
      Pages 61-70
    6. Mary J. C. Hendrix, Elisabeth A. Seftor, Richard E. B. Seftor
      Pages 71-79
    7. Marc E. Bracke, Tom Boterberg, Erik A. Bruyneel, Marc M. Mareel
      Pages 81-89
    8. Marc E. Bracke, Tom Boterberg, Marc M. Mareel
      Pages 91-102
    9. Adam Jones, Chisato Fujiyama, Stephen Hague, Roy Bicknell
      Pages 119-124
    10. Pratima Nangia-Makker, Yuichiro Honjo, Avraham Raz
      Pages 149-157
  4. Animal Models of Metastasis

    1. Front Matter
      Pages 159-159
    2. Eric Petitclerc, Peter C. Brooks
      Pages 173-187
    3. Ruth J. Muschel, Jin Hua
      Pages 189-193
    4. Janet E. Price
      Pages 205-213
    5. Udo Schumacher, Susan A. Brooks
      Pages 215-222
    6. Raffaella Giavazzi, Angela Garofalo
      Pages 223-229
    7. Chantale T. Guy, Gizela Cardoso
      Pages 231-249
    8. Robert M. Hoffman
      Pages 285-298
  5. Back Matter
    Pages 299-308

About this book


Although ninety percent of fatal cancer cases involve the spread of a primary tumor, the formation of metastases is still a poorly understood, complex process and a significant problem in the treatment of cancer patients. In Metastasis Research Protocols, leading international investigators describe in detail the key methods needed to investigate why and how metastasis occurs. Volume II of this two-volume set, Analysis of Cell Behavior In Vitro and In Vivo, presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and in whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade. These systems include cell migration assays, assays for matrix degradation enzymes, basement membrane degradation assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models for metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis. The first volume of this set, Analysis of Cells and Tissues, focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression.
Comprehensive and authoritative, the two volumes of Metastasis Research Protocols constitute a gold-standard collection of readily reproducible methods for understanding the metastatic cascade-first at the cellular and molecular levels, then at the level of the whole organism-responsible for the spread of cancer and for developing novel strategies to combat its spread.

Editors and affiliations

  • Susan A. Brooks
    • 1
  • Udo Schumacher
    • 2
  1. 1.Research School of Biological and Molecular SciencesOxford Brookes UniversityOxfordUK
  2. 2.Institute for Anatomy, Department of NeuroanatomyUniversity Hospital Hamburg-EppendorfHamburgGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-615-4
  • Online ISBN 978-1-59259-137-4
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site